2gyk: Difference between revisions
New page: left|200px<br /><applet load="2gyk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gyk, resolution 1.600Å" /> '''Crystal structure o... |
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[[Image:2gyk.jpg|left|200px]]<br /><applet load="2gyk" size=" | [[Image:2gyk.jpg|left|200px]]<br /><applet load="2gyk" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2gyk, resolution 1.600Å" /> | caption="2gyk, resolution 1.600Å" /> | ||
'''Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)'''<br /> | '''Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2GYK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] Full crystallographic information is available from [http:// | 2GYK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:53 2008'' | ||
Revision as of 16:15, 23 January 2008
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Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)
OverviewOverview
The crystal structure of the cytotoxic endonuclease domain from the, bacterial toxin colicin E9 in complex with its cognate immunity protein, Im9 reveals that the inhibitor does not bind at the active site, the core, of which comprises the HNH motif found in intron-encoded homing, endonucleases, but rather at an adjacent position leaving the active site, exposed yet unable to bind DNA because of steric and electrostatic clashes, with incoming substrate. Although its mode of action is unorthodox, Im9 is, a remarkably effective inhibitor since it folds within milliseconds and, then associates with its target endonuclease at the rate of diffusion to, form an inactive complex with sub-femtomolar binding affinity. This, hyperefficient mechanism of inhibition could be well suited to other toxic, enzyme systems, particularly where the substrate is a polymer extending, beyond the boundaries of the active site.
About this StructureAbout this Structure
2GYK is a Protein complex structure of sequences from Escherichia coli with and as ligands. Active as Deoxyribonuclease I, with EC number 3.1.21.1 Full crystallographic information is available from OCA.
ReferenceReference
Structural and mechanistic basis of immunity toward endonuclease colicins., Kleanthous C, Kuhlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR, James R, Hemmings AM, Nat Struct Biol. 1999 Mar;6(3):243-52. PMID:10074943
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