2fk6: Difference between revisions
New page: left|200px<br /><applet load="2fk6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fk6, resolution 2.90Å" /> '''Crystal Structure of... |
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[[Image:2fk6.gif|left|200px]]<br /><applet load="2fk6" size=" | [[Image:2fk6.gif|left|200px]]<br /><applet load="2fk6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fk6, resolution 2.90Å" /> | caption="2fk6, resolution 2.90Å" /> | ||
'''Crystal Structure of RNAse Z/tRNA(Thr) complex'''<br /> | '''Crystal Structure of RNAse Z/tRNA(Thr) complex'''<br /> | ||
==Overview== | ==Overview== | ||
The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic | The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage. | ||
==About this Structure== | ==About this Structure== | ||
2FK6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with ZN, MES and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http:// | 2FK6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MES:'>MES</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mathy, N.]] | [[Category: Mathy, N.]] | ||
[[Category: Pellegrini, O.]] | [[Category: Pellegrini, O.]] | ||
[[Category: Sierra-Gallay, I | [[Category: Sierra-Gallay, I Li de la.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MES]] | [[Category: MES]] | ||
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[[Category: protein-trna complex; zinc-dependent metal hydrolase; trna maturase]] | [[Category: protein-trna complex; zinc-dependent metal hydrolase; trna maturase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:22:11 2008'' | ||
Revision as of 18:22, 21 February 2008
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Crystal Structure of RNAse Z/tRNA(Thr) complex
OverviewOverview
The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage.
About this StructureAbout this Structure
2FK6 is a Protein complex structure of sequences from Bacillus subtilis with , and as ligands. Active as Ribonuclease Z, with EC number 3.1.26.11 Full crystallographic information is available from OCA.
ReferenceReference
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA., Li de la Sierra-Gallay I, Mathy N, Pellegrini O, Condon C, Nat Struct Mol Biol. 2006 Apr;13(4):376-7. Epub 2006 Mar 5. PMID:16518398
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