2fa2: Difference between revisions

Revision as of 18:19, 21 February 2008

Crystal structure of Fus3 without a peptide from Ste5

OverviewOverview

Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.

About this StructureAbout this Structure

2FA2 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.

ReferenceReference

The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway., Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA, Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19. PMID:16424299

Page seeded by OCA on Thu Feb 21 17:19:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2fa2.gif|left|200px]]<br /><applet load="2fa2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fa2.gif|left|200px]]<br /><applet load="2fa2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fa2, resolution 2.85&Aring;" />
 '''Crystal structure of Fus3 without a peptide from Ste5'''<br />
 ==Overview==
-Scaffold proteins organize signaling proteins into pathways and are often, viewed as passive assembly platforms. We found that the Ste5 scaffold has, a more active role in the yeast mating pathway: A fragment of Ste5, allosterically activated autophosphorylation of the mitogen-activated, protein kinase Fus3. The resulting form of Fus3 is partially active-it is, phosphorylated on only one of two key residues in the activation loop., Unexpectedly, at a systems level, autoactivated Fus3 appears to have a, negative regulatory role, promoting Ste5 phosphorylation and a decrease in, pathway transcriptional output. Thus, scaffolds not only direct basic, pathway connectivity but can precisely tune quantitative pathway, input-output properties.
+Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.
 ==About this Structure==
-FA2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SCN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FA2 OCA].
+FA2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SCN:'>SCN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FA2 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Bashor, C.J.]]
+[[Category: Bashor, C J.]]
-[[Category: Bhattacharyya, R.P.]]
+[[Category: Bhattacharyya, R P.]]
-[[Category: Falick, A.M.]]
+[[Category: Falick, A M.]]
-[[Category: Good, M.C.]]
+[[Category: Good, M C.]]
-[[Category: Lim, W.A.]]
+[[Category: Lim, W A.]]
 [[Category: Remenyi, A.]]
 [[Category: SCN]]
 [[Category: map kinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:26:50 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:20 2008''