2f68: Difference between revisions

Revision as of 18:18, 21 February 2008

Crystal structure of collagen adhesin (CNA) from S. aureus

OverviewOverview

The structural basis for the association of eukaryotic and prokaryotic protein receptors and their triple-helical collagen ligand remains poorly understood. Here, we present the crystal structures of a high affinity subsegment of the Staphylococcus aureus collagen-binding CNA as an apo-protein and in complex with a synthetic collagen-like triple helical peptide. The apo-protein structure is composed of two subdomains (N1 and N2), each adopting a variant IgG-fold, and a long linker that connects N1 and N2. The structure is stabilized by hydrophobic inter-domain interactions and by the N2 C-terminal extension that complements a beta-sheet on N1. In the ligand complex, the collagen-like peptide penetrates through a spherical hole formed by the two subdomains and the N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely designed to allow multidomain collagen binding proteins to bind their extended rope-like ligand.

About this StructureAbout this Structure

2F68 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen., Zong Y, Xu Y, Liang X, Keene DR, Hook A, Gurusiddappa S, Hook M, Narayana SV, EMBO J. 2005 Dec 21;24(24):4224-36. Epub 2005 Dec 15. PMID:16362049

Page seeded by OCA on Thu Feb 21 17:18:07 2008

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OCA

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-[[Image:2f68.gif|left|200px]]<br /><applet load="2f68" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2f68.gif|left|200px]]<br /><applet load="2f68" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2f68, resolution 1.95&Aring;" />
 '''Crystal structure of collagen adhesin (CNA) from S. aureus'''<br />
 ==Overview==
-The structural basis for the association of eukaryotic and prokaryotic, protein receptors and their triple-helical collagen ligand remains poorly, understood. Here, we present the crystal structures of a high affinity, subsegment of the Staphylococcus aureus collagen-binding CNA as an, apo-protein and in complex with a synthetic collagen-like triple helical, peptide. The apo-protein structure is composed of two subdomains (N1 and, N2), each adopting a variant IgG-fold, and a long linker that connects N1, and N2. The structure is stabilized by hydrophobic inter-domain, interactions and by the N2 C-terminal extension that complements a, beta-sheet on N1. In the ligand complex, the collagen-like peptide, penetrates through a spherical hole formed by the two subdomains and the, N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely, designed to allow multidomain collagen binding proteins to bind their, extended rope-like ligand.
+The structural basis for the association of eukaryotic and prokaryotic protein receptors and their triple-helical collagen ligand remains poorly understood. Here, we present the crystal structures of a high affinity subsegment of the Staphylococcus aureus collagen-binding CNA as an apo-protein and in complex with a synthetic collagen-like triple helical peptide. The apo-protein structure is composed of two subdomains (N1 and N2), each adopting a variant IgG-fold, and a long linker that connects N1 and N2. The structure is stabilized by hydrophobic inter-domain interactions and by the N2 C-terminal extension that complements a beta-sheet on N1. In the ligand complex, the collagen-like peptide penetrates through a spherical hole formed by the two subdomains and the N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely designed to allow multidomain collagen binding proteins to bind their extended rope-like ligand.
 ==About this Structure==
-F68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA].
+F68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Narayana, S.V.L.]]
+[[Category: Narayana, S V.L.]]
 [[Category: Zong, Y.]]
 [[Category: beta barrel]]
 [[Category: domain swap]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:23:15 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:07 2008''