2f01: Difference between revisions
New page: left|200px<br /><applet load="2f01" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f01, resolution 0.85Å" /> '''Epi-biotin complex w... |
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[[Image:2f01.gif|left|200px]]<br /><applet load="2f01" size=" | [[Image:2f01.gif|left|200px]]<br /><applet load="2f01" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2f01, resolution 0.85Å" /> | caption="2f01, resolution 0.85Å" /> | ||
'''Epi-biotin complex with core streptavidin'''<br /> | '''Epi-biotin complex with core streptavidin'''<br /> | ||
==Overview== | ==Overview== | ||
(+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral | (+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral center at atom C2. This could lead to a difference in the mode of binding of (+)-epi-biotin to streptavidin, a natural protein receptor for (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom resolution for structural analysis of the complex of streptavidin with a sample of (+)-epi-biotin and refinement was carried out at both 1.0 and 0.85 Angstrom resolution. The structure determination shows a superposition of two ligands in the binding site, (+)-biotin and (+)-epi-biotin. The molecules overlap in the model for the complex except for the position of S1 in the tetrahydrothiophene ring. Differences in the conformation of the ring permits binding of each molecule to streptavidin with little observable difference in the protein structures at this high resolution. | ||
==About this Structure== | ==About this Structure== | ||
2F01 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with BTN, BTQ and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2F01 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=BTN:'>BTN</scene>, <scene name='pdbligand=BTQ:'>BTQ</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F01 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces avidinii]] | [[Category: Streptomyces avidinii]] | ||
[[Category: Aubert, D | [[Category: Aubert, D G.]] | ||
[[Category: Stenkamp, R | [[Category: Stenkamp, R E.]] | ||
[[Category: Thomas, N | [[Category: Thomas, N R.]] | ||
[[Category: Trong, I | [[Category: Trong, I Le.]] | ||
[[Category: BTN]] | [[Category: BTN]] | ||
[[Category: BTQ]] | [[Category: BTQ]] | ||
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[[Category: streptavidin]] | [[Category: streptavidin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:20 2008'' | ||
Revision as of 18:16, 21 February 2008
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Epi-biotin complex with core streptavidin
OverviewOverview
(+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral center at atom C2. This could lead to a difference in the mode of binding of (+)-epi-biotin to streptavidin, a natural protein receptor for (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom resolution for structural analysis of the complex of streptavidin with a sample of (+)-epi-biotin and refinement was carried out at both 1.0 and 0.85 Angstrom resolution. The structure determination shows a superposition of two ligands in the binding site, (+)-biotin and (+)-epi-biotin. The molecules overlap in the model for the complex except for the position of S1 in the tetrahydrothiophene ring. Differences in the conformation of the ring permits binding of each molecule to streptavidin with little observable difference in the protein structures at this high resolution.
About this StructureAbout this Structure
2F01 is a Single protein structure of sequence from Streptomyces avidinii with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:16699183
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