1h9c: Difference between revisions

Revision as of 15:15, 30 October 2007

NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.

OverviewOverview

The determination by NMR of the solution structure of the phosphorylated, enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific, phosphoenolpyruvate-dependent phosphotransferase system of Escherichia, coli is presented. Most of the backbone and side-chain resonances were, assigned using a variety of mostly heteronuclear NMR experiments. The, remaining resonances were assigned with the help of the structure, calculations.NOE-derived distance restraints were used in distance, geometry calculations followed by molecular dynamics and simulated, annealing protocols. In addition, combinations of ambiguous restraints, were used to resolve ambiguities in the NOE assignments. By combining sets, of ambiguous and unambiguous restraints into new ambiguous restraints, an, error function was ... [(full description)]

About this StructureAbout this Structure

1H9C is a [Single protein] structure of sequence from [Escherichia coli]. Active as [Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [2.7.1.69]. Structure known Active Sites: PBL and PBS. Full crystallographic information is available from [OCA].

ReferenceReference

NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:11352587

Page seeded by OCA on Tue Oct 30 14:20:33 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==About this Structure==
-H9C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]].
+H9C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]]. Structure known Active Sites: PBL and PBS. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]].
 ==Reference==
 NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11352587 11352587]
 [[Category: Escherichia coli]]
+[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
 [[Category: Single protein]]
 [[Category: Ab, E.]]
@@ Line 28: / Line 29: @@
 [[Category: transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:52:47 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:20:33 2007''