2bn5: Difference between revisions

Revision as of 17:39, 21 February 2008

P-ELEMENT SOMATIC INHIBITOR PROTEIN COMPLEX WITH U1-70K PROLINE-RICH PEPTIDE

OverviewOverview

P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.

About this StructureAbout this Structure

2BN5 is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112

Page seeded by OCA on Thu Feb 21 16:39:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2bn5.gif|left|200px]]<br /><applet load="2bn5" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bn5.gif|left|200px]]<br /><applet load="2bn5" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2bn5" />
 '''P-ELEMENT SOMATIC INHIBITOR PROTEIN COMPLEX WITH U1-70K PROLINE-RICH PEPTIDE'''<br />
 ==Overview==
-P-element transposition in Drosophila is regulated by tissue-specific, alternative splicing of the P-element transposase pre-mRNA. In somatic, cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of, the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to, the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the, genuine 5' splice site, thereby preventing excision of the third intron., Two homologous short sequences, referred to as the A and B boxes, near the, C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now, mapped the AB box-binding site of U1-70k to a short proline-rich sequence, at the C terminus. Our NMR study shows that the B box forms an, anti-parallel helical hairpin in which four highly conserved aromatic, residues form a cluster on one face of the first helix. This hydrophobic, cluster interacts extensively with the proline-rich region of the U1-70k, protein.
+P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
 ==About this Structure==
-BN5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA].
+BN5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Drosophila melanogaster]]
 [[Category: Protein complex]]
-[[Category: Butler, P.J.G.]]
+[[Category: Butler, P J.G.]]
 [[Category: Ignjatovic, T.]]
 [[Category: Nagai, K.]]
 [[Category: Neuhaus, D.]]
-[[Category: Yang, J.C.]]
+[[Category: Yang, J C.]]
 [[Category: complex]]
 [[Category: inhibitor]]
@@ Line 26: / Line 26: @@
 [[Category: splicing]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:50:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:32 2008''