2aut: Difference between revisions
New page: left|200px<br /><applet load="2aut" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aut, resolution 2.25Å" /> '''Crystal structure of... |
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[[Image:2aut.jpg|left|200px]]<br /><applet load="2aut" size=" | [[Image:2aut.jpg|left|200px]]<br /><applet load="2aut" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2aut, resolution 2.25Å" /> | caption="2aut, resolution 2.25Å" /> | ||
'''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''<br /> | '''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''<br /> | ||
==Overview== | ==Overview== | ||
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was | The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules. | ||
==About this Structure== | ==About this Structure== | ||
2AUT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG, NA and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http:// | 2AUT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUT OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gupta, G | [[Category: Gupta, G D.]] | ||
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
[[Category: Makde, R | [[Category: Makde, R D.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: metalloenzyme]] | [[Category: metalloenzyme]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:16 2008'' | ||
Revision as of 17:31, 21 February 2008
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Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium
OverviewOverview
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
About this StructureAbout this Structure
2AUT is a Single protein structure of sequence from Salmonella typhimurium with , and as ligands. Active as Acid phosphatase, with EC number 3.1.3.2 Full crystallographic information is available from OCA.
ReferenceReference
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
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