1vsk: Difference between revisions
New page: left|200px<br /> <applet load="1vsk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vsk, resolution 2.2Å" /> '''ASV INTEGRASE CORE D... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1VSK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VSK OCA]]. | 1VSK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]]. Active as [[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VSK OCA]]. | ||
==Reference== | ==Reference== | ||
Structural basis for inactivating mutations and pH-dependent activity of avian sarcoma virus integrase., Lubkowski J, Yang F, Alexandratos J, Merkel G, Katz RA, Gravuer K, Skalka AM, Wlodawer A, J Biol Chem. 1998 Dec 4;273(49):32685-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9830010 9830010] | Structural basis for inactivating mutations and pH-dependent activity of avian sarcoma virus integrase., Lubkowski J, Yang F, Alexandratos J, Merkel G, Katz RA, Gravuer K, Skalka AM, Wlodawer A, J Biol Chem. 1998 Dec 4;273(49):32685-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9830010 9830010] | ||
[[Category: RNA-directed DNA polymerase]] | |||
[[Category: Rous sarcoma virus]] | [[Category: Rous sarcoma virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 23: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:14:24 2007'' | ||
Revision as of 15:09, 30 October 2007
|
ASV INTEGRASE CORE DOMAIN D64N MUTATION IN CITRATE BUFFER PH 6.0
OverviewOverview
Crystallographic studies of the catalytic core domain of avian sarcoma, virus integrase (ASV IN) have provided the most detailed picture so far of, the active site of this enzyme, which belongs to an important class of, targets for designing drugs against AIDS. Recently, crystals of an, inactive D64N mutant were obtained under conditions identical to those, used for the native enzyme. Data were collected at different pH values and, in the presence of divalent cations. Data were also collected at low pH, for the crystals of the native ASV IN core domain. In the structures of, native ASV IN at pH 6.0 and below, as well as in all structures of the, D64N mutants, the side chain of the active site residue Asx-64 (Asx, denotes Asn or Asp) is rotated by approximately 150 degrees around the, ... [(full description)]
About this StructureAbout this Structure
1VSK is a [Single protein] structure of sequence from [Rous sarcoma virus]. Active as [RNA-directed DNA polymerase], with EC number [2.7.7.49]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
ReferenceReference
Structural basis for inactivating mutations and pH-dependent activity of avian sarcoma virus integrase., Lubkowski J, Yang F, Alexandratos J, Merkel G, Katz RA, Gravuer K, Skalka AM, Wlodawer A, J Biol Chem. 1998 Dec 4;273(49):32685-9. PMID:9830010
Page seeded by OCA on Tue Oct 30 14:14:24 2007