2ahm: Difference between revisions
New page: left|200px<br /><applet load="2ahm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ahm, resolution 2.4Å" /> '''Crystal structure of ... |
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[[Image:2ahm.gif|left|200px]]<br /><applet load="2ahm" size=" | [[Image:2ahm.gif|left|200px]]<br /><applet load="2ahm" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2ahm, resolution 2.4Å" /> | caption="2ahm, resolution 2.4Å" /> | ||
'''Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer'''<br /> | '''Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer'''<br /> | ||
==Overview== | ==Overview== | ||
Coronavirus replication and transcription machinery involves multiple | Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7-nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-angstroms resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of approximately 30 angstroms, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase. | ||
==About this Structure== | ==About this Structure== | ||
2AHM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2AHM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Rao, Z.]] | [[Category: Rao, Z.]] | ||
[[Category: Sun, F.]] | [[Category: Sun, F.]] | ||
[[Category: Zhai, Y | [[Category: Zhai, Y J.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: super-complex]] | [[Category: super-complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:28 2008'' | ||
Revision as of 17:27, 21 February 2008
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Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer
OverviewOverview
Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7-nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-angstroms resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of approximately 30 angstroms, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase.
About this StructureAbout this Structure
2AHM is a Protein complex structure of sequences from Human sars coronavirus with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer., Zhai Y, Sun F, Li X, Pang H, Xu X, Bartlam M, Rao Z, Nat Struct Mol Biol. 2005 Nov;12(11):980-6. PMID:16228002
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