2aa9: Difference between revisions

Revision as of 17:25, 21 February 2008

EPSP synthase liganded with shikimate

OverviewOverview

The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.

About this StructureAbout this Structure

2AA9 is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as 3-phosphoshikimate 1-carboxyvinyltransferase, with EC number 2.5.1.19 Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867

Page seeded by OCA on Thu Feb 21 16:25:27 2008

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OCA

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-[[Image:2aa9.gif|left|200px]]<br /><applet load="2aa9" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2aa9.gif|left|200px]]<br /><applet load="2aa9" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2aa9, resolution 1.5&Aring;" />
 '''EPSP synthase liganded with shikimate'''<br />
 ==Overview==
-The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase, (EPSP synthase) has received attention in the past because it is the, target of the broad-spectrum herbicide glyphosate. The natural substrate, of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also, utilize shikimate as substrate. Remarkably, this reaction is insensitive, to inhibition by glyphosate. Crystallographic analysis of EPSP synthase, from Escherichia coli, in complex with shikimate/glyphosate at 1.5, Angstroms resolution, revealed that binding of shikimate induces changes, around the backbone of the active site, which in turn impact the efficient, binding of glyphosate. The implications from these findings with respect, to the design of novel glyphosate-insensitive EPSP synthase enzymes are, discussed.
+The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.
 ==About this Structure==
-AA9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SKM and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AA9 OCA].
+AA9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SKM:'>SKM</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AA9 OCA].
 ==Reference==
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 [[Category: Becker, A.]]
 [[Category: Funke, T.]]
-[[Category: Healy, M.L.]]
+[[Category: Healy, M L.]]
-[[Category: Priestman, M.A.]]
+[[Category: Priestman, M A.]]
 [[Category: Schonbrunn, E.]]
 [[Category: FMT]]
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 [[Category: inside-out alpha/beta barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:00:41 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:27 2008''