2a7c: Difference between revisions

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On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength

OverviewOverview

Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.

About this StructureAbout this Structure

2A7C is a Single protein structure of sequence from Sus scrofa with , and as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.

ReferenceReference

On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength., Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1263-72. Epub 2005, Aug 16. PMID:16131760

Page seeded by OCA on Thu Feb 21 16:24:17 2008

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OCA

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-[[Image:2a7c.gif|left|200px]]<br /><applet load="2a7c" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2a7c.gif|left|200px]]<br /><applet load="2a7c" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2a7c, resolution 1.65&Aring;" />
 '''On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength'''<br />
 ==Overview==
-Complete and highly redundant data sets were collected at different, wavelengths between 0.80 and 2.65 A for a total of ten different protein, and DNA model systems. The magnitude of the anomalous signal-to-noise, ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be, influenced by the data-collection wavelength and the nature of the, anomalously scattering substructure. By utilizing simple empirical, correlations, for instance between the estimated deltaF/F and the expected, R(anom) or the data-collection wavelength and the expected R(r.i.m.), the, wavelength at which the highest anomalous signal-to-noise ratio can be, expected could be estimated even before the experiment. Almost independent, of the nature of the anomalously scattering substructure and provided that, no elemental X-ray absorption edge is nearby, this optimal wavelength is, 2.1 A.
+Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.
 ==About this Structure==
-A7C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4, XE and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A7C OCA].
+A7C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=XE:'>XE</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7C OCA].
 ==Reference==
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 [[Category: Mueller-Dieckmann, C.]]
 [[Category: Panjikar, S.]]
-[[Category: Tucker, P.A.]]
+[[Category: Tucker, P A.]]
-[[Category: Weiss, M.S.]]
+[[Category: Weiss, M S.]]
 [[Category: GOL]]
 [[Category: SO4]]
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 [[Category: hydrolase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:57:41 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:17 2008''