1z3e: Difference between revisions
New page: left|200px<br /><applet load="1z3e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z3e, resolution 1.500Å" /> '''Crystal Structure o... |
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[[Image:1z3e.gif|left|200px]]<br /><applet load="1z3e" size=" | [[Image:1z3e.gif|left|200px]]<br /><applet load="1z3e" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1z3e, resolution 1.500Å" /> | caption="1z3e, resolution 1.500Å" /> | ||
'''Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit'''<br /> | '''Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit'''<br /> | ||
==Overview== | ==Overview== | ||
Spx, a global transcription regulator in Bacillus subtilis, interacts with | Spx, a global transcription regulator in Bacillus subtilis, interacts with the C-terminal domain of the alpha subunit (alphaCTD) of RNA polymerase to control gene expression under conditions of disulfide stress, which is sensed by disulfide bond formation between Spx residues C10 and C13. Here, we describe the crystal structure of the B. subtilis alphaCTD bound to oxidized Spx. Analysis of the complex reveals interactions between three regions of "anti-alpha" Spx and helix alpha1 and the "261" determinant of alphaCTD. The former contact could disrupt the interaction between alphaCTD and activator proteins or alter the DNA-bound conformation of alphaCTD, thereby repressing activator-stimulated transcription. Binding to the 261 determinant would prevent interaction between alphaCTD and region 4 of sigma(A). Intriguingly, the Spx disulfide bond is far from the alphaCTD-Spx interface, suggesting that Spx regulates transcription allosterically or through the redox-dependent creation or destruction of binding sites for additional components of the transcription machinery. | ||
==About this Structure== | ==About this Structure== | ||
1Z3E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | 1Z3E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3E OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Brennan, R | [[Category: Brennan, R G.]] | ||
[[Category: Nakano, S.]] | [[Category: Nakano, S.]] | ||
[[Category: Newberry, K | [[Category: Newberry, K J.]] | ||
[[Category: Zuber, P.]] | [[Category: Zuber, P.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: disulfide stress]] | [[Category: disulfide stress]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:43 2008'' | ||
Revision as of 17:11, 21 February 2008
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Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit
OverviewOverview
Spx, a global transcription regulator in Bacillus subtilis, interacts with the C-terminal domain of the alpha subunit (alphaCTD) of RNA polymerase to control gene expression under conditions of disulfide stress, which is sensed by disulfide bond formation between Spx residues C10 and C13. Here, we describe the crystal structure of the B. subtilis alphaCTD bound to oxidized Spx. Analysis of the complex reveals interactions between three regions of "anti-alpha" Spx and helix alpha1 and the "261" determinant of alphaCTD. The former contact could disrupt the interaction between alphaCTD and activator proteins or alter the DNA-bound conformation of alphaCTD, thereby repressing activator-stimulated transcription. Binding to the 261 determinant would prevent interaction between alphaCTD and region 4 of sigma(A). Intriguingly, the Spx disulfide bond is far from the alphaCTD-Spx interface, suggesting that Spx regulates transcription allosterically or through the redox-dependent creation or destruction of binding sites for additional components of the transcription machinery.
About this StructureAbout this Structure
1Z3E is a Protein complex structure of sequences from Bacillus subtilis with as ligand. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase., Newberry KJ, Nakano S, Zuber P, Brennan RG, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15839-44. Epub 2005 Oct 25. PMID:16249335
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