1yk4: Difference between revisions

Revision as of 17:06, 21 February 2008

Ultra-high resolution structure of Pyrococcus abyssi rubredoxin W4L/R5S

OverviewOverview

The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was solved by direct methods. The model of the air-oxidized protein was refined by partially restrained full-matrix least-squares refinement against intensity data to 0.69 A resolution. This first ultrahigh-resolution structure of a rubredoxin provides very detailed and precise information about the Fe(SCys)(4) centre and its environment, the peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and dynamic disorder, the solvent structure and the electron-density distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of mutants studied by atomic and ultrahigh-resolution X-ray crystallography which are expected to contribute to the understanding of structure-function relationships in iron-sulfur proteins.

About this StructureAbout this Structure

1YK4 is a Single protein structure of sequence from Pyrococcus abyssi with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin. I. 0.69 A X-ray structure of mutant W4L/R5S., Bonisch H, Schmidt CL, Bianco P, Ladenstein R, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):990-1004. Epub 2005, Jun 24. PMID:15983423

Page seeded by OCA on Thu Feb 21 16:06:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1yk4.gif|left|200px]]<br /><applet load="1yk4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1yk4.gif|left|200px]]<br /><applet load="1yk4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1yk4, resolution 0.69&Aring;" />
 '''Ultra-high resolution structure of Pyrococcus abyssi rubredoxin W4L/R5S'''<br />
 ==Overview==
-The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was, solved by direct methods. The model of the air-oxidized protein was, refined by partially restrained full-matrix least-squares refinement, against intensity data to 0.69 A resolution. This first, ultrahigh-resolution structure of a rubredoxin provides very detailed and, precise information about the Fe(SCys)(4) centre and its environment, the, peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and, dynamic disorder, the solvent structure and the electron-density, distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of, mutants studied by atomic and ultrahigh-resolution X-ray crystallography, which are expected to contribute to the understanding of, structure-function relationships in iron-sulfur proteins.
+The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was solved by direct methods. The model of the air-oxidized protein was refined by partially restrained full-matrix least-squares refinement against intensity data to 0.69 A resolution. This first ultrahigh-resolution structure of a rubredoxin provides very detailed and precise information about the Fe(SCys)(4) centre and its environment, the peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and dynamic disorder, the solvent structure and the electron-density distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of mutants studied by atomic and ultrahigh-resolution X-ray crystallography which are expected to contribute to the understanding of structure-function relationships in iron-sulfur proteins.
 ==About this Structure==
-YK4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YK4 OCA].
+YK4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with <scene name='pdbligand=FE:'>FE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK4 OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Bonisch, H.]]
 [[Category: Ladenstein, R.]]
-[[Category: Schmidt, C.L.]]
+[[Category: Schmidt, C L.]]
 [[Category: FE]]
 [[Category: electron transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:51:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:15 2008''