1qie: Difference between revisions
New page: left|200px<br /> <applet load="1qie" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qie, resolution 2.10Å" /> '''SPECIFIC CHEMICAL A... |
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==About this Structure== | ==About this Structure== | ||
1QIE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QIE OCA]]. | 1QIE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]]. Active as [[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QIE OCA]]. | ||
==Reference== | ==Reference== | ||
Specific chemical and structural damage to proteins produced by synchrotron radiation., Weik M, Ravelli RB, Kryger G, McSweeney S, Raves ML, Harel M, Gros P, Silman I, Kroon J, Sussman JL, Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):623-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10639129 10639129] | Specific chemical and structural damage to proteins produced by synchrotron radiation., Weik M, Ravelli RB, Kryger G, McSweeney S, Raves ML, Harel M, Gros P, Silman I, Kroon J, Sussman JL, Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):623-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10639129 10639129] | ||
[[Category: Acetylcholinesterase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Torpedo californica]] | [[Category: Torpedo californica]] | ||
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[[Category: time series]] | [[Category: time series]] | ||
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Revision as of 14:58, 30 October 2007
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SPECIFIC CHEMICAL AND STRUCTURAL DAMAGE AT NINE TIME POINTS (POINT B) CAUSED BY INTENSE SYNCHROTRON RADIATION TO TORPEDO CALIFORNICA ACETYLCHOLINESTERASE
OverviewOverview
Radiation damage is an inherent problem in x-ray crystallography. It, usually is presumed to be nonspecific and manifested as a gradual decay in, the overall quality of data obtained for a given crystal as data, collection proceeds. Based on third-generation synchrotron x-ray data, collected at cryogenic temperatures, we show for the enzymes Torpedo, californica acetylcholinesterase and hen egg white lysozyme that, synchrotron radiation also can cause highly specific damage. Disulfide, bridges break, and carboxyl groups of acidic residues lose their, definition. Highly exposed carboxyls, and those in the active site of both, enzymes, appear particularly susceptible. The catalytic triad residue, His-440, in acetylcholinesterase, also appears to be much more sensitive, to radiation damage ... [(full description)]
About this StructureAbout this Structure
1QIE is a [Single protein] structure of sequence from [Torpedo californica]. Active as [Acetylcholinesterase], with EC number [3.1.1.7]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
ReferenceReference
Specific chemical and structural damage to proteins produced by synchrotron radiation., Weik M, Ravelli RB, Kryger G, McSweeney S, Raves ML, Harel M, Gros P, Silman I, Kroon J, Sussman JL, Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):623-8. PMID:10639129
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