1xa9: Difference between revisions
New page: left|200px<br /><applet load="1xa9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xa9, resolution 2.5Å" /> '''Crystal structure of ... |
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[[Image:1xa9.gif|left|200px]]<br /><applet load="1xa9" size=" | [[Image:1xa9.gif|left|200px]]<br /><applet load="1xa9" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1xa9, resolution 2.5Å" /> | caption="1xa9, resolution 2.5Å" /> | ||
'''Crystal structure of yellow fluorescent protein zFP538 K66M green mutant'''<br /> | '''Crystal structure of yellow fluorescent protein zFP538 K66M green mutant'''<br /> | ||
==Overview== | ==Overview== | ||
Crystal structures of the tetrameric yellow-fluorescent protein zFP538 | Crystal structures of the tetrameric yellow-fluorescent protein zFP538 from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are presented. The atomic models have been refined at 2.7 and 2.5 A resolution, with final crystallographic R factors of 0.206 (R(free) = 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent stereochemistry. The fold of the protomer is very similar to that of green (GFP) and red (DsRed) fluorescent proteins; however, evidence from crystallography and mass spectrometry suggests that zFP538 contains a three-ring chromophore derived from that of GFP. The yellow-emitting species (lambda(em)(max) = 538 nm) is proposed to result from a transimination reaction in which a transiently appearing DsRed-like acylimine is attacked by the terminal amino group of lysine 66 to form a new six-membered ring, cleaving the polypeptide backbone at the 65-66 position. This extends the chromophore conjugation by an additional double bond compared to GFP, lowering the absorption and emission frequencies. Substitution of lysine 66 with aspartate or glutamate partially converts zFP538 into a red-fluorescent protein, providing additional support for an acylimine intermediate. The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins. | ||
==About this Structure== | ==About this Structure== | ||
1XA9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1XA9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Zoanthus sp.]] | [[Category: Zoanthus sp.]] | ||
[[Category: Anderson, D | [[Category: Anderson, D C.]] | ||
[[Category: Branchaud, B.]] | [[Category: Branchaud, B.]] | ||
[[Category: Kallio, K.]] | [[Category: Kallio, K.]] | ||
[[Category: Lukyanov, K | [[Category: Lukyanov, K A.]] | ||
[[Category: Remington, S | [[Category: Remington, S J.]] | ||
[[Category: Wachter, R | [[Category: Wachter, R M.]] | ||
[[Category: Yarbrough, D | [[Category: Yarbrough, D K.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
[[Category: beta-barrel]] | [[Category: beta-barrel]] | ||
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[[Category: fluorescent protein]] | [[Category: fluorescent protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:40 2008'' | ||
Revision as of 16:52, 21 February 2008
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Crystal structure of yellow fluorescent protein zFP538 K66M green mutant
OverviewOverview
Crystal structures of the tetrameric yellow-fluorescent protein zFP538 from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are presented. The atomic models have been refined at 2.7 and 2.5 A resolution, with final crystallographic R factors of 0.206 (R(free) = 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent stereochemistry. The fold of the protomer is very similar to that of green (GFP) and red (DsRed) fluorescent proteins; however, evidence from crystallography and mass spectrometry suggests that zFP538 contains a three-ring chromophore derived from that of GFP. The yellow-emitting species (lambda(em)(max) = 538 nm) is proposed to result from a transimination reaction in which a transiently appearing DsRed-like acylimine is attacked by the terminal amino group of lysine 66 to form a new six-membered ring, cleaving the polypeptide backbone at the 65-66 position. This extends the chromophore conjugation by an additional double bond compared to GFP, lowering the absorption and emission frequencies. Substitution of lysine 66 with aspartate or glutamate partially converts zFP538 into a red-fluorescent protein, providing additional support for an acylimine intermediate. The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins.
About this StructureAbout this Structure
1XA9 is a Single protein structure of sequence from Zoanthus sp. with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore., Remington SJ, Wachter RM, Yarbrough DK, Branchaud B, Anderson DC, Kallio K, Lukyanov KA, Biochemistry. 2005 Jan 11;44(1):202-12. PMID:15628861
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