User:Ralf Stephan/Sandbox 2: Difference between revisions

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'''Metalloproteases''' are [http://en.wikipedia.org/wiki/Proteases proteases] where the water molecule that is used for hydrolysis is complexed to a metal ion in the catalytic center of the enzyme. The metal ion is itself hold in position by several amino acid residues. The metalloproteases are one of seven superclans of the proteases in the UniProt/MEROPS classification.
[[Image:2a7g.png|left|200px]]


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== Classification ==
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A first raw classification is that into exo- and endopeptidases, according to whether the protein is cleaved at the end or not. The most advanced classification is that edited by UniProt and available at the MEROPS database (see link below). It lists 54 families of closely related metalloproteases that are grouped into 15 clans. We have chosen six examples from different clans that can be viewed by clicking on the green links:
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{{STRUCTURE_2a7g|  PDB=2a7g  |  SCENE=  }}


== Classification and catalytic center ==
*neutral zinc metallopeptidases, example thermolysin from ''Bac. stearothermophilus'' ([[2a7g]]). Matrix metallopeptidases and a lot of well-known other proteases belong to this big clan.
Thermolysin is a well researched [http://en.wikipedia.org/wiki/Metallopeptidase metalloprotease] containing <scene name='User:Ralf_Stephan/Sandbox_2/Zinc/2'>zinc</scene> and the amino acids His-Glu-X-His-His as its catalytic center. <scene name='User:Ralf_Stephan/Sandbox_2/Res_yellow/3'>Glu-166, His-142 and -146 are grouped around the zinc atom</scene>, holding it fast, while <scene name='User:Ralf_Stephan/Sandbox_2/Res/1'>Glu-143 holds the polarized water atom. Additionally, Tyr-157 and His-231</scene> stabilize the substrate protein which will be cleaved into two smaller proteins.<ref>Matthews, BW. (1988): ''Structural basis of the action of thermolysin and related zinc peptidases''. In: ''Acc. Chem. Res.'' '''21'''(9); 333–340; http://dx.doi.org/10.1021/ar00153a003</ref><ref>PMID:11935352</ref>
*carboxypeptidases, example carboxypeptidase A (''Bos taurus'', [[5cpa]])
*insulinases, example pitrilysin (''E. coli'', [[1q2l]])
*cytosol-aminopeptidases, example leucyl aminopeptidase (''Bos taurus'', [[1lam]])
*methionin-aminopeptidases, example human methionine aminopeptidase ([[2b3k]])
*renal dipeptidases, example human renal dipeptidase ([[1itq]])


==About this Structure==
== Weblinks ==
2A7G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7G OCA].
*[http://merops.sanger.ac.uk/cgi-bin/make_clan_index?type=peptidase MEROPS: Clans of peptidases]
 
==On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength==
 
 
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==References==
<references />
 
[[Category: Bacillus thermoproteolyticus]]
[[Category: Single protein]]
[[Category: Transaldolase]]
[[Category: Mueller-Dieckmann, C.]]
[[Category: Panjikar, S.]]
[[Category: Tucker, P A.]]
[[Category: Weiss, M S.]]
[[Category: Transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:20:02 2008''

Revision as of 19:28, 9 February 2009

Metalloproteases are proteases where the water molecule that is used for hydrolysis is complexed to a metal ion in the catalytic center of the enzyme. The metal ion is itself hold in position by several amino acid residues. The metalloproteases are one of seven superclans of the proteases in the UniProt/MEROPS classification.

ClassificationClassification

A first raw classification is that into exo- and endopeptidases, according to whether the protein is cleaved at the end or not. The most advanced classification is that edited by UniProt and available at the MEROPS database (see link below). It lists 54 families of closely related metalloproteases that are grouped into 15 clans. We have chosen six examples from different clans that can be viewed by clicking on the green links:

  • neutral zinc metallopeptidases, example thermolysin from Bac. stearothermophilus (2a7g). Matrix metallopeptidases and a lot of well-known other proteases belong to this big clan.
  • carboxypeptidases, example carboxypeptidase A (Bos taurus, 5cpa)
  • insulinases, example pitrilysin (E. coli, 1q2l)
  • cytosol-aminopeptidases, example leucyl aminopeptidase (Bos taurus, 1lam)
  • methionin-aminopeptidases, example human methionine aminopeptidase (2b3k)
  • renal dipeptidases, example human renal dipeptidase (1itq)

WeblinksWeblinks

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