1wac: Difference between revisions

Revision as of 16:42, 21 February 2008

BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE

OverviewOverview

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

About this StructureAbout this Structure

1WAC is a Single protein structure of sequence from Bacteriophage phi-6. Full crystallographic information is available from OCA.

ReferenceReference

Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773

Page seeded by OCA on Thu Feb 21 15:42:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1wac.gif|left|200px]]<br /><applet load="1wac" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wac.gif|left|200px]]<br /><applet load="1wac" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1wac, resolution 3.0&Aring;" />
 '''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''<br />
 ==Overview==
-The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage, phi6 is capable of primer-independent initiation, as are many RNA, polymerases. The structure of this polymerase revealed an initiation, platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been, identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical, studies have addressed the role of this platform, revealing that a mutant, version can utilize a back-priming initiation mechanism, where the 3', terminus of the template adopts a hairpin-like conformation. Here, the, mechanism of back-primed initiation is studied further by biochemical and, structural methods.
+The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
 ==About this Structure==
-WAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
+WAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
 ==Reference==
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 [[Category: Bacteriophage phi-6]]
 [[Category: Single protein]]
-[[Category: Bamford, D.H.]]
+[[Category: Bamford, D H.]]
-[[Category: Grimes, J.M.]]
+[[Category: Grimes, J M.]]
-[[Category: Laurila, M.R.L.]]
+[[Category: Laurila, M R.L.]]
-[[Category: Salgado, P.S.]]
+[[Category: Salgado, P S.]]
-[[Category: Stuart, D.I.]]
+[[Category: Stuart, D I.]]
 [[Category: phi6 rna-dependent rna polymerase]]
 [[Category: transcription]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:16:39 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:08 2008''