1v8z: Difference between revisions

Revision as of 16:32, 21 February 2008

X-ray crystal structure of the Tryptophan Synthase b2 Subunit from Hyperthermophile, Pyrococcus furiosus

OverviewOverview

The structure of the tryptophan synthase beta2 subunit (Pfbeta2) from the hyperthermophile, Pyrococcus furiosus, was determined by X-ray crystallographic analysis at 2.2 A resolution, and its stability was examined by DSC. This is the first report of the X-ray structure of the tryptophan synthase beta2 subunit alone, although the structure of the tryptophan synthase alpha2beta2 complex from Salmonella typhimurium has already been reported. The structure of Pfbeta2 was essentially similar to that of the beta2 subunit (Stbeta2) in the alpha2beta2 complex from S. typhimurium. The sequence alignment with secondary structures of Pfbeta and Stbeta in monomeric form showed that six residues in the N-terminal region and three residues in the C-terminal region were deleted in Pfbeta, and one residue at Pro366 of Stbeta and at Ile63 of Pfbeta was inserted. The denaturation temperature of Pfbeta2 was higher by 35 degrees C than the reported values from mesophiles at approximately pH 8. On the basis of structural information on both proteins, the analyses of the contributions of each stabilization factor indicate that: (a) the higher stability of Pfbeta2 is not caused by either a hydrophobic interaction or an increase in ion pairs; (b) the number of hydrogen bonds involved in the main chains of Pfbeta is greater by about 10% than that of Stbeta, indicating that the secondary structures of Pfbeta are more stabilized than those of Stbeta and (c) the sequence of Pfbeta seems to be better fitted to an ideally stable structure than that of Stbeta, as assessed from X-ray structure data.

About this StructureAbout this Structure

1V8Z is a Single protein structure of sequence from Pyrococcus furiosus with and as ligands. Active as Tryptophan synthase, with EC number 4.2.1.20 Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of the tryptophan synthase beta subunit from the hyperthermophile Pyrococcus furiosus. Investigation of stabilization factors., Hioki Y, Ogasahara K, Lee SJ, Ma J, Ishida M, Yamagata Y, Matsuura Y, Ota M, Ikeguchi M, Kuramitsu S, Yutani K, Eur J Biochem. 2004 Jul;271(13):2624-35. PMID:15206928

Page seeded by OCA on Thu Feb 21 15:32:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1v8z.gif|left|200px]]<br /><applet load="1v8z" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1v8z.gif|left|200px]]<br /><applet load="1v8z" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1v8z, resolution 2.21&Aring;" />
 '''X-ray crystal structure of the Tryptophan Synthase b2 Subunit from Hyperthermophile, Pyrococcus furiosus'''<br />
 ==Overview==
-The structure of the tryptophan synthase beta2 subunit (Pfbeta2) from the, hyperthermophile, Pyrococcus furiosus, was determined by X-ray, crystallographic analysis at 2.2 A resolution, and its stability was, examined by DSC. This is the first report of the X-ray structure of the, tryptophan synthase beta2 subunit alone, although the structure of the, tryptophan synthase alpha2beta2 complex from Salmonella typhimurium has, already been reported. The structure of Pfbeta2 was essentially similar to, that of the beta2 subunit (Stbeta2) in the alpha2beta2 complex from S., typhimurium. The sequence alignment with secondary structures of Pfbeta, and Stbeta in monomeric form showed that six residues in the N-terminal, region and three residues in the C-terminal region were deleted in Pfbeta, and one residue at Pro366 of Stbeta and at Ile63 of Pfbeta was inserted., The denaturation temperature of Pfbeta2 was higher by 35 degrees C than, the reported values from mesophiles at approximately pH 8. On the basis of, structural information on both proteins, the analyses of the contributions, of each stabilization factor indicate that: (a) the higher stability of, Pfbeta2 is not caused by either a hydrophobic interaction or an increase, in ion pairs; (b) the number of hydrogen bonds involved in the main chains, of Pfbeta is greater by about 10% than that of Stbeta, indicating that the, secondary structures of Pfbeta are more stabilized than those of Stbeta, and (c) the sequence of Pfbeta seems to be better fitted to an ideally, stable structure than that of Stbeta, as assessed from X-ray structure, data.
+The structure of the tryptophan synthase beta2 subunit (Pfbeta2) from the hyperthermophile, Pyrococcus furiosus, was determined by X-ray crystallographic analysis at 2.2 A resolution, and its stability was examined by DSC. This is the first report of the X-ray structure of the tryptophan synthase beta2 subunit alone, although the structure of the tryptophan synthase alpha2beta2 complex from Salmonella typhimurium has already been reported. The structure of Pfbeta2 was essentially similar to that of the beta2 subunit (Stbeta2) in the alpha2beta2 complex from S. typhimurium. The sequence alignment with secondary structures of Pfbeta and Stbeta in monomeric form showed that six residues in the N-terminal region and three residues in the C-terminal region were deleted in Pfbeta, and one residue at Pro366 of Stbeta and at Ile63 of Pfbeta was inserted. The denaturation temperature of Pfbeta2 was higher by 35 degrees C than the reported values from mesophiles at approximately pH 8. On the basis of structural information on both proteins, the analyses of the contributions of each stabilization factor indicate that: (a) the higher stability of Pfbeta2 is not caused by either a hydrophobic interaction or an increase in ion pairs; (b) the number of hydrogen bonds involved in the main chains of Pfbeta is greater by about 10% than that of Stbeta, indicating that the secondary structures of Pfbeta are more stabilized than those of Stbeta and (c) the sequence of Pfbeta seems to be better fitted to an ideally stable structure than that of Stbeta, as assessed from X-ray structure data.
 ==About this Structure==
-V8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with NA and PLP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V8Z OCA].
+V8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V8Z OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: Ishida, M.]]
 [[Category: Kuramitsu, S.]]
-[[Category: Lee, S.J.]]
+[[Category: Lee, S J.]]
 [[Category: Ma, J.]]
 [[Category: Matsuura, Y.]]
 [[Category: Ogasahara, K.]]
 [[Category: Ota, M.]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Yamagata, Y.]]
 [[Category: Yutani, K.]]
@@ Line 32: / Line 32: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:30:52 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:32:46 2008''