Transcription Termination Factor Rho: Difference between revisions
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<font color='#20603c'>'''Arg347'''</font>, | <font color='#20603c'>'''Arg347'''</font>, | ||
<font color='#974e94'>'''Arg353'''</font>, and (in black) '''background RNA'''. | <font color='#974e94'>'''Arg353'''</font>, and (in black) '''background RNA'''. | ||
==Additional Resources== | |||
For additional information, see: [[Transcription and RNA Processing]] | |||
<br /> | |||
==Notes== | ==Notes== | ||
<references /> | <references /> | ||
Revision as of 15:04, 2 October 2010
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Transcription termination factor Rho is a ring-shaped RNA-DNA helicase that induces release of transcription complexes at specific loci of bacterial genomes. Several structures of Rho in various liganded states haven been solved by X-ray crystallography by the J. M. Berger lab (Berkeley): 1a8v, 1pv4, 1pvo, 1xpr, 1xpu, 2a8v, and 2ht1.
The asymmetric unit solved as 2ht1 contains only two chains, so it represents only one third of the biological unit, which is a homo-hexamer. The scene here shows this homo-hexamer in a closed state ()[1]. Note that this model has only the middle 324 amino acids in each chain, lacking the first 52 and the last 57 amino acids in each chain[2].
In this model (), the Protein hexamer is bound to RNA, and some incidental "Solvent" (sulfate ions).
Here is one representation showing the same Rho hexamer in a closed state and with . In this scene, the foreground RNA is colored by element (C O N P). Also colored are Lys181, Lys283, Arg347, Arg353, and (in black) background RNA.
Additional ResourcesAdditional Resources
For additional information, see: Transcription and RNA Processing
NotesNotes
- ↑ File File:Rho1.pdb derived from 2ht1 by symmetry operations
- ↑ Missing residues can be found by aligning the PDB file's SEQRES records with the sequence of the residues assigned atomic coordinates, as listed by the S2C Server