1tx7: Difference between revisions
New page: left|200px<br /><applet load="1tx7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tx7, resolution 1.75Å" /> '''Bovine Trypsin compl... |
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[[Image:1tx7.gif|left|200px]]<br /><applet load="1tx7" size=" | [[Image:1tx7.gif|left|200px]]<br /><applet load="1tx7" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1tx7, resolution 1.75Å" /> | caption="1tx7, resolution 1.75Å" /> | ||
'''Bovine Trypsin complexed with p-amidinophenylmethylphosphinic acid (AMPA)'''<br /> | '''Bovine Trypsin complexed with p-amidinophenylmethylphosphinic acid (AMPA)'''<br /> | ||
==Overview== | ==Overview== | ||
p-amidinophenylmethylphosphinic acid (AMPA) was designed, synthesized and | p-amidinophenylmethylphosphinic acid (AMPA) was designed, synthesized and crystallized in complex with trypsin to study interactions with the oxyanion hole at the S1 site. In comparison to benzamidine, AMPA shows improved activity, which the crystal structure demonstrates to result from hydrogen bonds between the negatively charged phosphinic acid group and the catalytic residues at the oxyanion hole. | ||
==About this Structure== | ==About this Structure== | ||
1TX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA and 4CM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http:// | 1TX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=4CM:'>4CM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TX7 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Cui, J.]] | [[Category: Cui, J.]] | ||
[[Category: Fu, W.]] | [[Category: Fu, W.]] | ||
[[Category: Johnson, M | [[Category: Johnson, M E.]] | ||
[[Category: Marankan, F.]] | [[Category: Marankan, F.]] | ||
[[Category: Mesecar, A.]] | [[Category: Mesecar, A.]] | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:17 2008'' | ||
Revision as of 16:18, 21 February 2008
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Bovine Trypsin complexed with p-amidinophenylmethylphosphinic acid (AMPA)
OverviewOverview
p-amidinophenylmethylphosphinic acid (AMPA) was designed, synthesized and crystallized in complex with trypsin to study interactions with the oxyanion hole at the S1 site. In comparison to benzamidine, AMPA shows improved activity, which the crystal structure demonstrates to result from hydrogen bonds between the negatively charged phosphinic acid group and the catalytic residues at the oxyanion hole.
About this StructureAbout this Structure
1TX7 is a Single protein structure of sequence from Bos taurus with and as ligands. Active as Trypsin, with EC number 3.4.21.4 Full crystallographic information is available from OCA.
ReferenceReference
An oxyanion-hole selective serine protease inhibitor in complex with trypsin., Cui J, Marankan F, Fu W, Crich D, Mesecar A, Johnson ME, Bioorg Med Chem. 2002 Jan;10(1):41-6. PMID:11738605
Page seeded by OCA on Thu Feb 21 15:18:17 2008