1stp: Difference between revisions

Revision as of 16:05, 21 February 2008

STRUCTURAL ORIGINS OF HIGH-AFFINITY BIOTIN BINDING TO STREPTAVIDIN

OverviewOverview

The high affinity of the noncovalent interaction between biotin and streptavidin forms the basis for many diagnostic assays that require the formation of an irreversible and specific linkage between biological macromolecules. Comparison of the refined crystal structures of apo and a streptavidin:biotin complex shows that the high affinity results from several factors. These factors include the formation of multiple hydrogen bonds and van der Waals interactions between biotin and the protein, together with the ordering of surface polypeptide loops that bury the biotin in the protein interior. Structural alterations at the biotin binding site produce quaternary changes in the streptavidin tetramer. These changes apparently propagate through cooperative deformations in the twisted beta sheets that link tetramer subunits.

About this StructureAbout this Structure

1STP is a Single protein structure of sequence from Streptomyces avidinii with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structural origins of high-affinity biotin binding to streptavidin., Weber PC, Ohlendorf DH, Wendoloski JJ, Salemme FR, Science. 1989 Jan 6;243(4887):85-8. PMID:2911722

Page seeded by OCA on Thu Feb 21 15:05:03 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1stp.gif|left|200px]]<br /><applet load="1stp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1stp.gif|left|200px]]<br /><applet load="1stp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1stp, resolution 2.6&Aring;" />
 '''STRUCTURAL ORIGINS OF HIGH-AFFINITY BIOTIN BINDING TO STREPTAVIDIN'''<br />
 ==Overview==
-The high affinity of the noncovalent interaction between biotin and, streptavidin forms the basis for many diagnostic assays that require the, formation of an irreversible and specific linkage between biological, macromolecules. Comparison of the refined crystal structures of apo and a, streptavidin:biotin complex shows that the high affinity results from, several factors. These factors include the formation of multiple hydrogen, bonds and van der Waals interactions between biotin and the protein, together with the ordering of surface polypeptide loops that bury the, biotin in the protein interior. Structural alterations at the biotin, binding site produce quaternary changes in the streptavidin tetramer., These changes apparently propagate through cooperative deformations in the, twisted beta sheets that link tetramer subunits.
+The high affinity of the noncovalent interaction between biotin and streptavidin forms the basis for many diagnostic assays that require the formation of an irreversible and specific linkage between biological macromolecules. Comparison of the refined crystal structures of apo and a streptavidin:biotin complex shows that the high affinity results from several factors. These factors include the formation of multiple hydrogen bonds and van der Waals interactions between biotin and the protein, together with the ordering of surface polypeptide loops that bury the biotin in the protein interior. Structural alterations at the biotin binding site produce quaternary changes in the streptavidin tetramer. These changes apparently propagate through cooperative deformations in the twisted beta sheets that link tetramer subunits.
 ==About this Structure==
-STP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with BTN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1STP OCA].
+STP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=BTN:'>BTN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STP OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Streptomyces avidinii]]
-[[Category: Salemme, F.R.]]
+[[Category: Salemme, F R.]]
-[[Category: Weber, P.C.]]
+[[Category: Weber, P C.]]
 [[Category: BTN]]
 [[Category: biotin binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:39:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:03 2008''