1rxq: Difference between revisions
New page: left|200px<br /><applet load="1rxq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rxq, resolution 1.70Å" /> '''YfiT from Bacillus s... |
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[[Image:1rxq.gif|left|200px]]<br /><applet load="1rxq" size=" | [[Image:1rxq.gif|left|200px]]<br /><applet load="1rxq" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rxq, resolution 1.70Å" /> | caption="1rxq, resolution 1.70Å" /> | ||
'''YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology'''<br /> | '''YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology'''<br /> | ||
==Overview== | ==Overview== | ||
YfiT, a 19-kDa polypeptide from Bacillus subtilis, belongs to a small | YfiT, a 19-kDa polypeptide from Bacillus subtilis, belongs to a small sequence family with members predominantly from Gram positive bacteria. We have determined the crystal structure of YfiT in complex with Ni(2+) to a resolution of 1.7 A. YfiT exists as a dimer and binds Ni(2+) in a 1:1 stoichiometry. The protein has an unusual four-helix bundle topology and coordinates Ni(2+) in an octahedral geometry with three conserved histidines and three waters. Although there is no similarity in their overall structures, the coordination geometry of the metal and the residues that constitute the putative active site in YfiT are similar to those of metalloproteases such as thermolysin. Our structural analyses suggest that YfiT might function as a metal-dependent hydrolase. | ||
==About this Structure== | ==About this Structure== | ||
1RXQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with NI and GLU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1RXQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=GLU:'>GLU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Anderson, W | [[Category: Anderson, W F.]] | ||
[[Category: MCSG, Midwest | [[Category: MCSG, Midwest Center for Structural Genomics.]] | ||
[[Category: Rajan, S | [[Category: Rajan, S S.]] | ||
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
[[Category: GLU]] | [[Category: GLU]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:39 2008'' | ||
Revision as of 15:55, 21 February 2008
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YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology
OverviewOverview
YfiT, a 19-kDa polypeptide from Bacillus subtilis, belongs to a small sequence family with members predominantly from Gram positive bacteria. We have determined the crystal structure of YfiT in complex with Ni(2+) to a resolution of 1.7 A. YfiT exists as a dimer and binds Ni(2+) in a 1:1 stoichiometry. The protein has an unusual four-helix bundle topology and coordinates Ni(2+) in an octahedral geometry with three conserved histidines and three waters. Although there is no similarity in their overall structures, the coordination geometry of the metal and the residues that constitute the putative active site in YfiT are similar to those of metalloproteases such as thermolysin. Our structural analyses suggest that YfiT might function as a metal-dependent hydrolase.
About this StructureAbout this Structure
1RXQ is a Single protein structure of sequence from Bacillus subtilis with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology., Rajan SS, Yang X, Shuvalova L, Collart F, Anderson WF, Biochemistry. 2004 Dec 14;43(49):15472-9. PMID:15581359
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