1rio: Difference between revisions
New page: left|200px<br /><applet load="1rio" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rio, resolution 2.30Å" /> '''Structure of bacteri... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1rio.gif|left|200px]]<br /><applet load="1rio" size=" | [[Image:1rio.gif|left|200px]]<br /><applet load="1rio" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rio, resolution 2.30Å" /> | caption="1rio, resolution 2.30Å" /> | ||
'''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA'''<br /> | '''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA'''<br /> | ||
==Overview== | ==Overview== | ||
The cI protein of bacteriophage lambda (lambdacI) activates transcription | The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process. | ||
==About this Structure== | ==About this Structure== | ||
1RIO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] and [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1RIO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] and [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIO OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
[[Category: Darst, S | [[Category: Darst, S A.]] | ||
[[Category: Hochschild, A.]] | [[Category: Hochschild, A.]] | ||
[[Category: Jain, D.]] | [[Category: Jain, D.]] | ||
[[Category: Nickels, B | [[Category: Nickels, B E.]] | ||
[[Category: Sun, L.]] | [[Category: Sun, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 24: | Line 24: | ||
[[Category: transcription activation]] | [[Category: transcription activation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:17 2008'' | ||
Revision as of 15:51, 21 February 2008
|
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
OverviewOverview
The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.
About this StructureAbout this Structure
1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393
Page seeded by OCA on Thu Feb 21 14:51:17 2008