1rg0: Difference between revisions
New page: left|200px<br /><applet load="1rg0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rg0, resolution 1.80Å" /> '''Monoclinic crystal f... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1rg0.gif|left|200px]]<br /><applet load="1rg0" size=" | [[Image:1rg0.gif|left|200px]]<br /><applet load="1rg0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rg0, resolution 1.80Å" /> | caption="1rg0, resolution 1.80Å" /> | ||
'''Monoclinic crystal form of the truncated K122-4 pilin from Pseudomonas aeruginosa'''<br /> | '''Monoclinic crystal form of the truncated K122-4 pilin from Pseudomonas aeruginosa'''<br /> | ||
==Overview== | ==Overview== | ||
Adherence of pathogens to host cells is critical for the initiation of | Adherence of pathogens to host cells is critical for the initiation of infection and is thus an attractive target for anti-infective therapeutics and vaccines. In the opportunistic human pathogen Pseudomonas aeruginosa, host-cell adherence is achieved predominantly by type IV pili. Analysis of several clinical strains of P. aeruginosa reveals poor sequence conservation between pilin genes, including the residues in the receptor-binding site. Interestingly, the receptor-binding sites appear to retain a conserved surface epitope because all Pseudomonas type IV pili recognize the same receptor on the host cell and cross-reactive antibodies specific for the receptor-binding site exist. Here, we present the crystallographic analysis of two crystal forms of truncated pilin from P. aeruginosa strain K122-4 (DeltaK122-4) at 1.54 and 1.8 A resolution, respectively. The DeltaK122-4 structure is compared to other crystallographically determined type IV pilin structures and an NMR structure of DeltaK122-4 pilin. A comparison with the structure of the highly divergent P. aeruginosa strain K (DeltaPAK) pilin indicates that the receptor-binding loop in both pilins forms a shallow depression with a surface that is formed by main-chain atoms. Conservation of this putative binding site is independent of the sequence as long as the main-chain conformation is conserved and could therefore explain the shared receptor specificity and antibody cross reactivity of highly divergent Pseudomonas type IV pilins. | ||
==About this Structure== | ==About this Structure== | ||
1RG0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http:// | 1RG0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RG0 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Audette, G | [[Category: Audette, G F.]] | ||
[[Category: Hazes, B.]] | [[Category: Hazes, B.]] | ||
[[Category: Irvin, R | [[Category: Irvin, R T.]] | ||
[[Category: adhesin]] | [[Category: adhesin]] | ||
[[Category: lectin]] | [[Category: lectin]] | ||
| Line 21: | Line 21: | ||
[[Category: type iv pilin]] | [[Category: type iv pilin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:30 2008'' | ||
