Pyruvate phosphate dikinase: Difference between revisions
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Pyruvate phosphate dikinase (PPDK) catalyzes the inter-conversion of adenosine triphosphate (ATP), P<sub>i</sub>, and pyruvate with adenine monophosphate (AMP), pyrophosphate (PP<sub>i</sub>, and phosphoenolpyruvate (PEP) in the presence of Mg<sup>2+</sup> and K<sup>+</sup>/Na<sup>+</sup>. The three-step reversible reaction proceeds via phosphoenzyme and pyrophosphoenzyme intermediates with a histidine residue serving as the phosphocarrier: | Pyruvate phosphate dikinase (PPDK) catalyzes the inter-conversion of adenosine triphosphate (ATP), P<sub>i</sub>, and pyruvate with adenine monophosphate (AMP), pyrophosphate (PP<sub>i</sub>, and phosphoenolpyruvate (PEP) in the presence of Mg<sup>2+</sup> and K<sup>+</sup>/Na<sup>+</sup>. The three-step reversible reaction proceeds via phosphoenzyme and pyrophosphoenzyme intermediates with a histidine residue serving as the phosphocarrier: | ||
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1. PPDK-His + PEP ⇄ PPDK-His~PO3 + pyruvate | |||
2. PPDK-His~PO3 + P2O7 ⇄ PPDK-His~P2O7 + PO3 | |||
3. PPDK-His~P2O7 + AMP ⇄ PPDK-His + ATP | |||
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== (1) PPDK-His + PEP ⇄ PPDK-His~PO<sub>3</sub> + pyruvate == | == (1) PPDK-His + PEP ⇄ PPDK-His~PO<sub>3</sub> + pyruvate == | ||