1qdp: Difference between revisions

Revision as of 15:38, 21 February 2008

SOLUTION STRUCTURE OF ROBUSTOXIN, THE LETHAL NEUROTOXIN FROM THE FUNNEL WEB SPIDER ATRAX ROBUSTUS, NMR, 20 STRUCTURES

OverviewOverview

The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four disulphide bonds, the connectivities of which were determined from NMR data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a 1-4/2-6/3-7/5-8 pattern). The structure consists of a small three-stranded, anti-parallel beta-sheet and a series of interlocking gamma-turns at the C-terminus. It also contains a cystine knot, thus placing it in the inhibitor cystine knot motif family of structures, which includes the omega-conotoxins and a number of plant and animal toxins and protease inhibitors. Robustoxin contains three distinct charged patches on its surface, and an extended loop that includes several aromatic and non-polar residues. Both of these structural features may play a role in its binding to the voltage-gated sodium channel.

About this StructureAbout this Structure

1QDP is a Single protein structure of sequence from Atrax robustus. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of robustoxin, the lethal neurotoxin from the funnel-web spider Atrax robustus., Pallaghy PK, Alewood D, Alewood PF, Norton RS, FEBS Lett. 1997 Dec 15;419(2-3):191-6. PMID:9428632

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 '''SOLUTION STRUCTURE OF ROBUSTOXIN, THE LETHAL NEUROTOXIN FROM THE FUNNEL WEB SPIDER ATRAX ROBUSTUS, NMR, 20 STRUCTURES'''<br />
 ==Overview==
-The solution structure of robustoxin, the lethal neurotoxin from the, Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H, NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four, disulphide bonds, the connectivities of which were determined from NMR, data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a, 1-4/2-6/3-7/5-8 pattern). The structure consists of a small, three-stranded, anti-parallel beta-sheet and a series of interlocking, gamma-turns at the C-terminus. It also contains a cystine knot, thus, placing it in the inhibitor cystine knot motif family of structures, which, includes the omega-conotoxins and a number of plant and animal toxins and, protease inhibitors. Robustoxin contains three distinct charged patches on, its surface, and an extended loop that includes several aromatic and, non-polar residues. Both of these structural features may play a role in, its binding to the voltage-gated sodium channel.
+The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four disulphide bonds, the connectivities of which were determined from NMR data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a 1-4/2-6/3-7/5-8 pattern). The structure consists of a small three-stranded, anti-parallel beta-sheet and a series of interlocking gamma-turns at the C-terminus. It also contains a cystine knot, thus placing it in the inhibitor cystine knot motif family of structures, which includes the omega-conotoxins and a number of plant and animal toxins and protease inhibitors. Robustoxin contains three distinct charged patches on its surface, and an extended loop that includes several aromatic and non-polar residues. Both of these structural features may play a role in its binding to the voltage-gated sodium channel.
 ==About this Structure==
-QDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Atrax_robustus Atrax robustus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QDP OCA].
+QDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Atrax_robustus Atrax robustus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDP OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Alewood, D.]]
-[[Category: Alewood, P.F.]]
+[[Category: Alewood, P F.]]
-[[Category: Norton, R.S.]]
+[[Category: Norton, R S.]]
-[[Category: Pallaghy, P.K.]]
+[[Category: Pallaghy, P K.]]
 [[Category: atrax robustus]]
 [[Category: cystine knot]]
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 [[Category: venom]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:36:17 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:33 2008''