1pop: Difference between revisions
New page: left|200px<br /><applet load="1pop" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pop, resolution 2.1Å" /> '''X-RAY CRYSTALLOGRAPHI... |
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[[Image:1pop.gif|left|200px]]<br /><applet load="1pop" size=" | [[Image:1pop.gif|left|200px]]<br /><applet load="1pop" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1pop, resolution 2.1Å" /> | caption="1pop, resolution 2.1Å" /> | ||
'''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX'''<br /> | '''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional structure of the papain-leupeptin complex has been | The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 A (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25. | ||
==About this Structure== | ==About this Structure== | ||
1POP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ACE and MOH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http:// | 1POP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=MOH:'>MOH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: hydrolase(thiol protease)]] | [[Category: hydrolase(thiol protease)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:57 2008'' | ||
