1pdf: Difference between revisions

Revision as of 15:27, 21 February 2008

Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex

OverviewOverview

The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.

About this StructureAbout this Structure

1PDF is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of bacteriophage T4 baseplate., Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG, Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:12923574

Page seeded by OCA on Thu Feb 21 14:27:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1pdf.gif|left|200px]]<br /><applet load="1pdf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pdf.gif|left|200px]]<br /><applet load="1pdf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pdf" />
 '''Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex'''<br />
 ==Overview==
-The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.
+The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.
 ==About this Structure==
-PDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDF OCA].
+PDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDF OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Arisaka, F.]]
-[[Category: Chipman, P.R.]]
+[[Category: Chipman, P R.]]
 [[Category: Kanamaru, S.]]
-[[Category: Kostyuchenko, V.A.]]
+[[Category: Kostyuchenko, V A.]]
-[[Category: Leiman, P.G.]]
+[[Category: Leiman, P G.]]
-[[Category: Mesyanzhinov, V.V.]]
+[[Category: Mesyanzhinov, V V.]]
-[[Category: Raaij, M.J.van.]]
+[[Category: Raaij, M J.van.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
 [[Category: structural protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:42:32 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:37 2008''