1v07: Difference between revisions
New page: left|200px<br /> <applet load="1v07" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v07, resolution 1.70Å" /> '''CRYSTAL STRUCTURE O... |
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==About this Structure== | ==About this Structure== | ||
1V07 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]] with SO4, HEM and OXY as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V07 OCA]]. | 1V07 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]] with SO4, HEM and OXY as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V07 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxygen affinity of c.lacteus mini-hemoglobin]] | [[Category: oxygen affinity of c.lacteus mini-hemoglobin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:30:36 2007'' | ||
Revision as of 14:25, 30 October 2007
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CRYSTAL STRUCTURE OF THRE11VAL MUTANT OF THE NERVE TISSUE MINI-HEMOGLOBIN FROM THE NEMERTEAN WORM CEREBRATULUS LACTEUS
OverviewOverview
The mini-hemoglobin from Cerebratulus lacteus (CerHb) belongs to a class, of globins containing the polar Tyr-B10/Gln-E7 amino acid pair that, normally causes low rates of O2 dissociation and ultra-high O2 affinity, which suggest O2 sensing or NO scavenging functions. CerHb, however, has, high rates of O2 dissociation (kO2 = 200-600 s(-1)) and moderate O2, affinity (KO2) approximately 1 microm(-1)) as a result of a third polar, amino acid in its active site, Thr-E11. When Thr-E11 is replaced by Val, kO2 decreases 1000-fold and KO2 increases 130-fold at pH 7.0, 20 degrees, C. The mutation also shifts the stretching frequencies of both heme-bound, and photodissociated CO, indicating marked changes of the electrostatic, field at the active site. The crystal structure of Thr-E11 --> Val ... [(full description)]
About this StructureAbout this Structure
1V07 is a [Single protein] structure of sequence from [Cerebratulus lacteus] with SO4, HEM and OXY as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Thr-E11 regulates O2 affinity in Cerebratulus lacteus mini-hemoglobin., Pesce A, Nardini M, Ascenzi P, Geuens E, Dewilde S, Moens L, Bolognesi M, Riggs AF, Hale A, Deng P, Nienhaus GU, Olson JS, Nienhaus K, J Biol Chem. 2004 Aug 6;279(32):33662-72. Epub 2004 May 25. PMID:15161908
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