1ny4: Difference between revisions
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'''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''<br /> | '''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''<br /> | ||
==Overview== | ==Overview== | ||
We report NMR assignments and solution structure of the 71-residue 30S | We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution. | ||
==About this Structure== | ==About this Structure== | ||
1NY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http:// | 1NY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NY4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Acton, T | [[Category: Acton, T B.]] | ||
[[Category: Aramini, J | [[Category: Aramini, J M.]] | ||
[[Category: Cort, J | [[Category: Cort, J R.]] | ||
[[Category: Ho, C | [[Category: Ho, C K.]] | ||
[[Category: Huang, Y | [[Category: Huang, Y J.]] | ||
[[Category: Kennedy, M | [[Category: Kennedy, M A.]] | ||
[[Category: Montelione, G | [[Category: Montelione, G T.]] | ||
[[Category: NESG, Northeast | [[Category: NESG, Northeast Structural Genomics Consortium.]] | ||
[[Category: Shih, L | [[Category: Shih, L Y.]] | ||
[[Category: Xiao, R.]] | [[Category: Xiao, R.]] | ||
[[Category: autostructure]] | [[Category: autostructure]] | ||
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[[Category: ribosomal protein]] | [[Category: ribosomal protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:18 2008'' | ||
Revision as of 15:11, 21 February 2008
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Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.
OverviewOverview
We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.
About this StructureAbout this Structure
1NY4 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.
ReferenceReference
Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:14627742
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Pyrococcus horikoshii
- Single protein
- Acton, T B.
- Aramini, J M.
- Cort, J R.
- Ho, C K.
- Huang, Y J.
- Kennedy, M A.
- Montelione, G T.
- NESG, Northeast Structural Genomics Consortium.
- Shih, L Y.
- Xiao, R.
- Autostructure
- Jr19
- Nesg
- Nmr structure
- Northeast structural genomics consortium
- Protein structure initiative
- Psi
- Ribosomal protein