1nvt: Difference between revisions
New page: left|200px<br /><applet load="1nvt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nvt, resolution 2.35Å" /> '''Crystal structure of... |
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[[Image:1nvt.gif|left|200px]]<br /><applet load="1nvt" size=" | [[Image:1nvt.gif|left|200px]]<br /><applet load="1nvt" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nvt, resolution 2.35Å" /> | caption="1nvt, resolution 2.35Å" /> | ||
'''Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+'''<br /> | '''Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of Methanococcus jannaschii shikimate | The crystal structure of Methanococcus jannaschii shikimate 5-dehydrogenase (MjSDH) bound to the cofactor nicotinamide adenine dinucleotide phosphate (NADP) has been determined at 2.35 A resolution. Shikimate 5-dehydrogenase (SDH) is responsible for NADP-dependent catalysis of the fourth step in shikimate biosynthesis, which is essential for aromatic amino acid metabolism in bacteria, microbial eukaryotes, and plants. The structure of MjSDH is a compact alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively. A phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site. The structure reveals a topologically new domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization. Insights gained from homology modeling and sequence/structure comparisons suggest that the SDHs represent a unique class of dehydrogenases. The structure provides a framework for further investigation to discover and develop novel inhibitors targeting this essential enzyme. | ||
==About this Structure== | ==About this Structure== | ||
1NVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ZN and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] Full crystallographic information is available from [http:// | 1NVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVT OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Shikimate dehydrogenase]] | [[Category: Shikimate dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Padyana, A | [[Category: Padyana, A K.]] | ||
[[Category: NAP]] | [[Category: NAP]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:36 2008'' | ||
