1njm: Difference between revisions

Revision as of 15:06, 21 February 2008

The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM) and the antibiotic sparsomycin

OverviewOverview

Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.

About this StructureAbout this Structure

1NJM is a Protein complex structure of sequences from Deinococcus radiodurans with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression., Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A, Mol Cell. 2003 Jan;11(1):91-102. PMID:12535524

Page seeded by OCA on Thu Feb 21 14:06:47 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1njm.gif|left|200px]]<br /><applet load="1njm" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1njm.gif|left|200px]]<br /><applet load="1njm" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1njm, resolution 3.60&Aring;" />
 '''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM) and the antibiotic sparsomycin'''<br />
 ==Overview==
-Crystal structures of tRNA mimics complexed with the large ribosomal, subunit of Deinococcus radiodurans indicate that remote interactions, determine the precise orientation of tRNA in the peptidyl-transferase, center (PTC). The PTC tolerates various orientations of puromycin, derivatives and its flexibility allows the conformational rearrangements, required for peptide-bond formation. Sparsomycin binds to A2602 and alters, the PTC conformation. H69, the intersubunit-bridge connecting the PTC and, decoding site, may also participate in tRNA placement and translocation. A, spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of, symmetry identified within the PTC suggests a unified ribosomal machinery, for peptide-bond formation, A-to-P-site translocation, and entrance of, nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the, universality of this mechanism.
+Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
 ==About this Structure==
-NJM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with SPS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJM OCA].
+NJM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with <scene name='pdbligand=SPS:'>SPS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJM OCA].
 ==Reference==
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 [[Category: Bashan, A.]]
 [[Category: Berisio, R.]]
-[[Category: Hansen, H.A.]]
+[[Category: Hansen, H A.]]
-[[Category: Harms, J.M.]]
+[[Category: Harms, J M.]]
 [[Category: Schluenzen, F.]]
 [[Category: Yonath, A.]]
@@ Line 30: / Line 30: @@
 [[Category: trna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:19:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:47 2008''