1nh2: Difference between revisions

Revision as of 15:06, 21 February 2008

Crystal structure of a yeast TFIIA/TBP/DNA complex

OverviewOverview

RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.

About this StructureAbout this Structure

1NH2 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes., Bleichenbacher M, Tan S, Richmond TJ, J Mol Biol. 2003 Sep 26;332(4):783-93. PMID:12972251

Page seeded by OCA on Thu Feb 21 14:06:00 2008

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OCA

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-[[Image:1nh2.gif|left|200px]]<br /><applet load="1nh2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1nh2.gif|left|200px]]<br /><applet load="1nh2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1nh2, resolution 1.90&Aring;" />
 '''Crystal structure of a yeast TFIIA/TBP/DNA complex'''<br />
 ==Overview==
-RNA polymerase II-dependent transcription requires the assembly of a, multi-protein, preinitiation complex on core promoter elements., Transcription factor IID (TFIID) comprising the TATA box-binding protein, (TBP) and TBP-associated factors (TAFs) is responsible for promoter, recognition in this complex. Subsequent association of TFIIA and TFIIB, provides enhanced complex stability. TFIIA is required for transcriptional, stimulation by certain viral and cellular activators, and favors formation, of the preinitiation complex in the presence of repressor NC2. The X-ray, structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A, resolution, respectively, are presented here and seen to resemble each, other closely. The interactions made by human TFIIA with TBP and DNA, within and upstream of the TATA box, including those involving water, molecules, are described and compared to the yeast structure. Of, particular interest is a previously unobserved region of TFIIA that, extends the binding interface with TBP in the yeast, but not in the human, complex, and that further elucidates biochemical and genetic results.
+RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.
 ==About this Structure==
-NH2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NH2 OCA].
+NH2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH2 OCA].
 ==Reference==
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 [[Category: Saccharomyces cerevisiae]]
 [[Category: Bleichenbacher, M.]]
-[[Category: Richmond, T.J.]]
+[[Category: Richmond, T J.]]
 [[Category: Tan, S.]]
 [[Category: transcription/dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:14:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:00 2008''