1nes: Difference between revisions
New page: left|200px<br /><applet load="1nes" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nes, resolution 1.65Å" /> '''STRUCTURE OF THE PRO... |
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[[Image:1nes.jpg|left|200px]]<br /><applet load="1nes" size=" | [[Image:1nes.jpg|left|200px]]<br /><applet load="1nes" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nes, resolution 1.65Å" /> | caption="1nes, resolution 1.65Å" /> | ||
'''STRUCTURE OF THE PRODUCT COMPLEX OF ACETYL-ALA-PRO-ALA WITH PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION'''<br /> | '''STRUCTURE OF THE PRODUCT COMPLEX OF ACETYL-ALA-PRO-ALA WITH PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
A single crystal of porcine pancreatic elastase was mounted in a | A single crystal of porcine pancreatic elastase was mounted in a thin-walled capillary and allowed to react with acetyl-Ala-Pro-Ala-paranitroanalide. Diffraction data to 1.65 A resolution were measured and the isomorphous structure was solved from the difference Fourier map. The structure contains two surprises. Two molecules of the product: acetyl-Ala-Pro-Ala molecule are bound in the extended binding site. Both molecules are bound backwards with respect to the established mode of peptide binding. | ||
==About this Structure== | ==About this Structure== | ||
1NES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA, SO4 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http:// | 1NES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NES OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
[[Category: Cole, G | [[Category: Cole, G M.]] | ||
[[Category: Junior, E | [[Category: Junior, E F.Meyer.]] | ||
[[Category: Presta, L | [[Category: Presta, L G.]] | ||
[[Category: Radhakrishnan, R.]] | [[Category: Radhakrishnan, R.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
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[[Category: complex (serine protease/inhibitor)]] | [[Category: complex (serine protease/inhibitor)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:19 2008'' | ||
