1mts: Difference between revisions

Revision as of 14:58, 21 February 2008

FACTOR XA SPECIFIC INHIBITOR IN COMPLEX WITH BOVINE TRYPSIN

OverviewOverview

Crystal structures of DX9065a and a related bisamidino-aryl inhibitor specific for the blood-clotting factor Xa have been solved in complex with bovine beta-trypsin to a resolution of 1.9 A. Each inhibitor exhibits an extended conformation along the active site, in contrast to the compact folded structures observed for thrombin specific inhibitors. Few direct contacts (predominantly in the S1 pocket) are made between trypsin and the inhibitors. Transfer of the inhibitors to the active site of factor Xa suggests a three-site interaction: salt bridge formation at the base of the primary specificity pocket, extensive hydrophobic surface burial and a weak electrostatic interaction between the distal basic component of the inhibitor and an electronegative cavity of factor Xa formed by three backbone carbonyl oxygens. Additivity of these three interactions is the basis for the observed strong inhibition of factor Xa and provides a framework for the design of novel factor Xa inhibitors. A propionic acid group of the inhibitor would clash with the thrombin specific '60-insertion loop', thus conferring selectivity against thrombin.

About this StructureAbout this Structure

1MTS is a Single protein structure of sequence from Bos taurus with and as ligands. Active as Trypsin, with EC number 3.4.21.4 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of factor Xa specific inhibitors in complex with trypsin: structural grounds for inhibition of factor Xa and selectivity against thrombin., Stubbs MT, Huber R, Bode W, FEBS Lett. 1995 Nov 13;375(1-2):103-7. PMID:7498454

Page seeded by OCA on Thu Feb 21 13:58:57 2008

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OCA

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-[[Image:1mts.jpg|left|200px]]<br /><applet load="1mts" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1mts, resolution 1.9&Aring;" />
 '''FACTOR XA SPECIFIC INHIBITOR IN COMPLEX WITH BOVINE TRYPSIN'''<br />
 ==Overview==
-Crystal structures of DX9065a and a related bisamidino-aryl inhibitor, specific for the blood-clotting factor Xa have been solved in complex with, bovine beta-trypsin to a resolution of 1.9 A. Each inhibitor exhibits an, extended conformation along the active site, in contrast to the compact, folded structures observed for thrombin specific inhibitors. Few direct, contacts (predominantly in the S1 pocket) are made between trypsin and the, inhibitors. Transfer of the inhibitors to the active site of factor Xa, suggests a three-site interaction: salt bridge formation at the base of, the primary specificity pocket, extensive hydrophobic surface burial and a, weak electrostatic interaction between the distal basic component of the, inhibitor and an electronegative cavity of factor Xa formed by three, backbone carbonyl oxygens. Additivity of these three interactions is the, basis for the observed strong inhibition of factor Xa and provides a, framework for the design of novel factor Xa inhibitors. A propionic acid, group of the inhibitor would clash with the thrombin specific, '60-insertion loop', thus conferring selectivity against thrombin.
+Crystal structures of DX9065a and a related bisamidino-aryl inhibitor specific for the blood-clotting factor Xa have been solved in complex with bovine beta-trypsin to a resolution of 1.9 A. Each inhibitor exhibits an extended conformation along the active site, in contrast to the compact folded structures observed for thrombin specific inhibitors. Few direct contacts (predominantly in the S1 pocket) are made between trypsin and the inhibitors. Transfer of the inhibitors to the active site of factor Xa suggests a three-site interaction: salt bridge formation at the base of the primary specificity pocket, extensive hydrophobic surface burial and a weak electrostatic interaction between the distal basic component of the inhibitor and an electronegative cavity of factor Xa formed by three backbone carbonyl oxygens. Additivity of these three interactions is the basis for the observed strong inhibition of factor Xa and provides a framework for the design of novel factor Xa inhibitors. A propionic acid group of the inhibitor would clash with the thrombin specific '60-insertion loop', thus conferring selectivity against thrombin.
 ==About this Structure==
-MTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA and BX3 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MTS OCA].
+MTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=BX3:'>BX3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTS OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Trypsin]]
-[[Category: Stubbs, M.T.]]
+[[Category: Stubbs, M T.]]
 [[Category: BX3]]
 [[Category: CA]]
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 [[Category: serine proteinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:41:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:57 2008''