1mbk: Difference between revisions
New page: left|200px<br /><applet load="1mbk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mbk" /> '''MOUSE C-MYB DNA-BINDING DOMAIN REPEAT 3'''<b... |
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'''MOUSE C-MYB DNA-BINDING DOMAIN REPEAT 3'''<br /> | '''MOUSE C-MYB DNA-BINDING DOMAIN REPEAT 3'''<br /> | ||
==Overview== | ==Overview== | ||
The DNA-binding domain of c-Myb consists of three imperfect tandem repeats | The DNA-binding domain of c-Myb consists of three imperfect tandem repeats (R1, R2 and R3). The three repeats have similar overall architectures, each containing a helix-turn-helix variation motif. The three conserved tryptophans in each repeat participate in forming a hydrophobic core. Comparison of the three repeat structures indicated that cavities are found in the hydrophobic core of R2, which is thermally unstable. On complexation with DNA, the orientations of R2 and R3 are fixed by tight binding and their conformations are slightly changed. No significant changes occur in the chemical shifts of R1 consistent with its loose interaction with DNA. | ||
==About this Structure== | ==About this Structure== | ||
1MBK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1MBK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: protooncogene product]] | [[Category: protooncogene product]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:34 2008'' | ||
Revision as of 14:53, 21 February 2008
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MOUSE C-MYB DNA-BINDING DOMAIN REPEAT 3
OverviewOverview
The DNA-binding domain of c-Myb consists of three imperfect tandem repeats (R1, R2 and R3). The three repeats have similar overall architectures, each containing a helix-turn-helix variation motif. The three conserved tryptophans in each repeat participate in forming a hydrophobic core. Comparison of the three repeat structures indicated that cavities are found in the hydrophobic core of R2, which is thermally unstable. On complexation with DNA, the orientations of R2 and R3 are fixed by tight binding and their conformations are slightly changed. No significant changes occur in the chemical shifts of R1 consistent with its loose interaction with DNA.
About this StructureAbout this Structure
1MBK is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb., Ogata K, Morikawa S, Nakamura H, Hojo H, Yoshimura S, Zhang R, Aimoto S, Ametani Y, Hirata Z, Sarai A, et al., Nat Struct Biol. 1995 Apr;2(4):309-20. PMID:7796266
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