1m1g: Difference between revisions

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Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)

OverviewOverview

Microbial transcription modulator NusG interacts with RNA polymerase and termination factor rho, displaying striking functional homology to eukaryotic Spt5. The protein is also a translational regulator. We have determined crystal structures of Aquifex aeolicus NusG showing a modular design: an N-terminal RNP-like domain, a C-terminal element with a KOW sequence motif and a species-specific immunoglobulin-like fold. The structures reveal bona fide nucleic acid binding sites, and nucleic acid binding activities can be detected for NusG from three organisms and for the KOW element alone. A conserved KOW domain is defined as a new class of nucleic acid binding folds. This module is a close structural homolog of tudor protein-protein interaction motifs. Putative protein binding sites for the RNP and KOW domains can be deduced, which differ from the areas implicated in nucleic acid interactions. The results strongly argue that both protein and nucleic acid contacts are important for NusG's functions and that the factor can act as an adaptor mediating indirect protein-nucleic acid associations.

About this StructureAbout this Structure

1M1G is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of transcription factor NusG in light of its nucleic acid- and protein-binding activities., Steiner T, Kaiser JT, Marinkovic S, Huber R, Wahl MC, EMBO J. 2002 Sep 2;21(17):4641-53. PMID:12198166

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OCA

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 '''Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)'''<br />
 ==Overview==
-Microbial transcription modulator NusG interacts with RNA polymerase and, termination factor rho, displaying striking functional homology to, eukaryotic Spt5. The protein is also a translational regulator. We have, determined crystal structures of Aquifex aeolicus NusG showing a modular, design: an N-terminal RNP-like domain, a C-terminal element with a KOW, sequence motif and a species-specific immunoglobulin-like fold. The, structures reveal bona fide nucleic acid binding sites, and nucleic acid, binding activities can be detected for NusG from three organisms and for, the KOW element alone. A conserved KOW domain is defined as a new class of, nucleic acid binding folds. This module is a close structural homolog of, tudor protein-protein interaction motifs. Putative protein binding sites, for the RNP and KOW domains can be deduced, which differ from the areas, implicated in nucleic acid interactions. The results strongly argue that, both protein and nucleic acid contacts are important for NusG's functions, and that the factor can act as an adaptor mediating indirect, protein-nucleic acid associations.
+Microbial transcription modulator NusG interacts with RNA polymerase and termination factor rho, displaying striking functional homology to eukaryotic Spt5. The protein is also a translational regulator. We have determined crystal structures of Aquifex aeolicus NusG showing a modular design: an N-terminal RNP-like domain, a C-terminal element with a KOW sequence motif and a species-specific immunoglobulin-like fold. The structures reveal bona fide nucleic acid binding sites, and nucleic acid binding activities can be detected for NusG from three organisms and for the KOW element alone. A conserved KOW domain is defined as a new class of nucleic acid binding folds. This module is a close structural homolog of tudor protein-protein interaction motifs. Putative protein binding sites for the RNP and KOW domains can be deduced, which differ from the areas implicated in nucleic acid interactions. The results strongly argue that both protein and nucleic acid contacts are important for NusG's functions and that the factor can act as an adaptor mediating indirect protein-nucleic acid associations.
 ==About this Structure==
-M1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M1G OCA].
+M1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1G OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Huber, R.]]
-[[Category: Kaiser, J.T.]]
+[[Category: Kaiser, J T.]]
 [[Category: Marinkovic, S.]]
 [[Category: Steiner, T.]]
-[[Category: Wahl, M.C.]]
+[[Category: Wahl, M C.]]
 [[Category: antitermination]]
 [[Category: immunoglobulin fold]]
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 [[Category: transcription termination]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:03:42 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:34 2008''