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Crystal Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with 6-phosphoryl-IMP, GDP and Hadacidin

OverviewOverview

Prokaryotes have a single form of adenylosuccinate synthetase that controls the committed step of AMP biosynthesis, but vertebrates have two isozymes of the synthetase. The basic isozyme, which predominates in muscle, participates in the purine nucleotide cycle, has an active site conformation different from that of the Escherichia coli enzyme, and exhibits significant differences in ligand recognition. Crystalline complexes presented here of the recombinant basic isozyme from mouse show the following. GTP alone binds to the active site without inducing a conformational change. IMP in combination with an acetate anion induces major conformational changes and organizes the active site for catalysis. IMP, in the absence of GTP, binds to the GTP pocket of the synthetase. The combination of GTP and IMP results in the formation of a stable complex of 6-phosphoryl-IMP and GDP in the presence or absence of hadacidin. The response of the basic isozyme to GTP alone differs from that of synthetases from plants, and yet the conformation of the mouse basic and E. coli synthetases in their complexes with GDP, 6-phosphoryl-IMP, and hadacidin are nearly identical. Hence, reported differences in ligand recognition among synthetases probably arise from conformational variations observed in partially ligated enzymes.

About this StructureAbout this Structure

1LON is a Single protein structure of sequence from Mus musculus with , , and as ligands. Active as Adenylosuccinate synthase, with EC number 6.3.4.4 Full crystallographic information is available from OCA.

ReferenceReference

IMP, GTP, and 6-phosphoryl-IMP complexes of recombinant mouse muscle adenylosuccinate synthetase., Iancu CV, Borza T, Fromm HJ, Honzatko RB, J Biol Chem. 2002 Jul 26;277(30):26779-87. Epub 2002 May 9. PMID:12004071

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OCA

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-[[Image:1lon.gif|left|200px]]<br /><applet load="1lon" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1lon, resolution 2.1&Aring;" />
 '''Crystal Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with 6-phosphoryl-IMP, GDP and Hadacidin'''<br />
 ==Overview==
-Prokaryotes have a single form of adenylosuccinate synthetase that, controls the committed step of AMP biosynthesis, but vertebrates have two, isozymes of the synthetase. The basic isozyme, which predominates in, muscle, participates in the purine nucleotide cycle, has an active site, conformation different from that of the Escherichia coli enzyme, and, exhibits significant differences in ligand recognition. Crystalline, complexes presented here of the recombinant basic isozyme from mouse show, the following. GTP alone binds to the active site without inducing a, conformational change. IMP in combination with an acetate anion induces, major conformational changes and organizes the active site for catalysis., IMP, in the absence of GTP, binds to the GTP pocket of the synthetase. The, combination of GTP and IMP results in the formation of a stable complex of, 6-phosphoryl-IMP and GDP in the presence or absence of hadacidin. The, response of the basic isozyme to GTP alone differs from that of, synthetases from plants, and yet the conformation of the mouse basic and, E. coli synthetases in their complexes with GDP, 6-phosphoryl-IMP, and, hadacidin are nearly identical. Hence, reported differences in ligand, recognition among synthetases probably arise from conformational, variations observed in partially ligated enzymes.
+Prokaryotes have a single form of adenylosuccinate synthetase that controls the committed step of AMP biosynthesis, but vertebrates have two isozymes of the synthetase. The basic isozyme, which predominates in muscle, participates in the purine nucleotide cycle, has an active site conformation different from that of the Escherichia coli enzyme, and exhibits significant differences in ligand recognition. Crystalline complexes presented here of the recombinant basic isozyme from mouse show the following. GTP alone binds to the active site without inducing a conformational change. IMP in combination with an acetate anion induces major conformational changes and organizes the active site for catalysis. IMP, in the absence of GTP, binds to the GTP pocket of the synthetase. The combination of GTP and IMP results in the formation of a stable complex of 6-phosphoryl-IMP and GDP in the presence or absence of hadacidin. The response of the basic isozyme to GTP alone differs from that of synthetases from plants, and yet the conformation of the mouse basic and E. coli synthetases in their complexes with GDP, 6-phosphoryl-IMP, and hadacidin are nearly identical. Hence, reported differences in ligand recognition among synthetases probably arise from conformational variations observed in partially ligated enzymes.
 ==About this Structure==
-LON is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, IMO, HDA and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LON OCA].
+LON is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=IMO:'>IMO</scene>, <scene name='pdbligand=HDA:'>HDA</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LON OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Borza, T.]]
-[[Category: Fromm, H.J.]]
+[[Category: Fromm, H J.]]
-[[Category: Honzatko, R.B.]]
+[[Category: Honzatko, R B.]]
-[[Category: Iancu, C.V.]]
+[[Category: Iancu, C V.]]
 [[Category: GDP]]
 [[Category: HDA]]
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 [[Category: purine biosynthesis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:43:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:53 2008''