1kyq: Difference between revisions
New page: left|200px<br /><applet load="1kyq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kyq, resolution 2.2Å" /> '''Met8p: A bifunctional... |
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[[Image:1kyq.gif|left|200px]]<br /><applet load="1kyq" size=" | [[Image:1kyq.gif|left|200px]]<br /><applet load="1kyq" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kyq, resolution 2.2Å" /> | caption="1kyq, resolution 2.2Å" /> | ||
'''Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.'''<br /> | '''Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.'''<br /> | ||
==Overview== | ==Overview== | ||
Sirohaem is a tetrapyrrole-derived prosthetic group that is required for | Sirohaem is a tetrapyrrole-derived prosthetic group that is required for the essential assimilation of sulfur and nitrogen into all living systems as part of the sulfite and nitrite reductase systems. The final two steps in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a bifunctional enzyme that carries out both of these reactions. Here, we report the 2.2 A resolution crystal structure of Met8p, which adopts a novel fold that bears no resemblance to the previously determined structures of cobalt- or ferro-chelatases. Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes. | ||
==About this Structure== | ==About this Structure== | ||
1KYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1KYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KYQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Brindley, A | [[Category: Brindley, A A.]] | ||
[[Category: Hill, C | [[Category: Hill, C P.]] | ||
[[Category: Raux, E.]] | [[Category: Raux, E.]] | ||
[[Category: Schubert, H | [[Category: Schubert, H L.]] | ||
[[Category: Warren, M | [[Category: Warren, M J.]] | ||
[[Category: Wilson, K | [[Category: Wilson, K S.]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:24 2008'' | ||
