1kxl: Difference between revisions
New page: left|200px<br /><applet load="1kxl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kxl" /> '''Solution Structure of the Cdc13 DNA-binding ... |
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'''Solution Structure of the Cdc13 DNA-binding Domain in a Complex with Single-Stranded Telomeric DNA (DNA structure not modeled)'''<br /> | '''Solution Structure of the Cdc13 DNA-binding Domain in a Complex with Single-Stranded Telomeric DNA (DNA structure not modeled)'''<br /> | ||
==Overview== | ==Overview== | ||
The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a | The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes. | ||
==About this Structure== | ==About this Structure== | ||
1KXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | 1KXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Anderson, E | [[Category: Anderson, E M.]] | ||
[[Category: Mitton-Fry, R | [[Category: Mitton-Fry, R M.]] | ||
[[Category: Wuttke, D | [[Category: Wuttke, D S.]] | ||
[[Category: dna-binding domain]] | [[Category: dna-binding domain]] | ||
[[Category: ob fold]] | [[Category: ob fold]] | ||
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[[Category: telomere]] | [[Category: telomere]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:01 2008'' | ||
Revision as of 14:39, 21 February 2008
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Solution Structure of the Cdc13 DNA-binding Domain in a Complex with Single-Stranded Telomeric DNA (DNA structure not modeled)
OverviewOverview
The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes.
About this StructureAbout this Structure
1KXL is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Conserved structure for single-stranded telomeric DNA recognition., Mitton-Fry RM, Anderson EM, Hughes TR, Lundblad V, Wuttke DS, Science. 2002 Apr 5;296(5565):145-7. PMID:11935027
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