1k8k: Difference between revisions
New page: left|200px<br /><applet load="1k8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k8k, resolution 2.0Å" /> '''Crystal Structure of ... |
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[[Image:1k8k.gif|left|200px]]<br /><applet load="1k8k" size=" | [[Image:1k8k.gif|left|200px]]<br /><applet load="1k8k" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1k8k, resolution 2.0Å" /> | caption="1k8k, resolution 2.0Å" /> | ||
'''Crystal Structure of Arp2/3 Complex'''<br /> | '''Crystal Structure of Arp2/3 Complex'''<br /> | ||
==Overview== | ==Overview== | ||
We determined a crystal structure of bovine Arp2/3 complex, an assembly of | We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament. | ||
==About this Structure== | ==About this Structure== | ||
1K8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | 1K8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Choe, S.]] | [[Category: Choe, S.]] | ||
[[Category: Higgs, H | [[Category: Higgs, H N.]] | ||
[[Category: Kaiser, D | [[Category: Kaiser, D A.]] | ||
[[Category: Marchand, J | [[Category: Marchand, J B.]] | ||
[[Category: Pollard, T | [[Category: Pollard, T D.]] | ||
[[Category: Robinson, R | [[Category: Robinson, R C.]] | ||
[[Category: Turbedsky, K.]] | [[Category: Turbedsky, K.]] | ||
[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:15 2008'' | ||
Revision as of 14:31, 21 February 2008
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Crystal Structure of Arp2/3 Complex
OverviewOverview
We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.
About this StructureAbout this Structure
1K8K is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Arp2/3 complex., Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S, Pollard TD, Science. 2001 Nov 23;294(5547):1679-84. PMID:11721045
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