1jvo: Difference between revisions
New page: left|200px<br /><applet load="1jvo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvo, resolution 2.75Å" /> '''Azurin dimer, crossl... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size=" | [[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1jvo, resolution 2.75Å" /> | caption="1jvo, resolution 2.75Å" /> | ||
'''Azurin dimer, crosslinked via disulfide bridge'''<br /> | '''Azurin dimer, crosslinked via disulfide bridge'''<br /> | ||
==Overview== | ==Overview== | ||
The transfer of electrons between proteins is an essential step in | The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers. | ||
==About this Structure== | ==About this Structure== | ||
1JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Amsterdam, I | [[Category: Amsterdam, I M.C van.]] | ||
[[Category: Canters, G | [[Category: Canters, G W.]] | ||
[[Category: Cavazzini, D.]] | [[Category: Cavazzini, D.]] | ||
[[Category: Einsle, O.]] | [[Category: Einsle, O.]] | ||
[[Category: Merli, A.]] | [[Category: Merli, A.]] | ||
[[Category: Messerschmidt, A.]] | [[Category: Messerschmidt, A.]] | ||
[[Category: Rossi, G | [[Category: Rossi, G L.]] | ||
[[Category: Ubbink, M.]] | [[Category: Ubbink, M.]] | ||
[[Category: CU]] | [[Category: CU]] | ||
| Line 26: | Line 26: | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:14 2008'' | ||
Revision as of 14:27, 21 February 2008
|
Azurin dimer, crosslinked via disulfide bridge
OverviewOverview
The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers.
About this StructureAbout this Structure
1JVO is a Single protein structure of sequence from Pseudomonas aeruginosa with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Dramatic modulation of electron transfer in protein complexes by crosslinking., van Amsterdam IM, Ubbink M, Einsle O, Messerschmidt A, Merli A, Cavazzini D, Rossi GL, Canters GW, Nat Struct Biol. 2002 Jan;9(1):48-52. PMID:11740504
Page seeded by OCA on Thu Feb 21 13:27:14 2008