1jj1: Difference between revisions

Revision as of 14:23, 21 February 2008

CRYSTAL STRUCTURE OF ORTHORHOMBIC LYSOZYME GROWN AT PH 4.6 IN PRESENCE OF 5% SORBITOL

OverviewOverview

In order to elucidate the effect of stabilizing additives on the structure of proteins and the associated ordered water molecules in the hydration shell, the crystal structures of tetragonal lysozyme grown in the presence of sucrose, sorbitol and trehalose have been refined. Also refined are the structures of orthorhombic and monoclinic lysozyme grown under the conditions in which tetragonal lysozyme is normally grown. A comparison of the two sets of structures with the structure of native tetragonal lysozyme shows that the effect of the additives on the structure of the protein molecule is less than that of the normal minor changes associated with differences in molecular packing. Surprisingly, the same is true of the effect on the hydration shell, represented by the ordered water molecules attached to the protein. Thus, it appears that the cause of the stabilizing effect of the additives needs to be sought outside the immediate neighbourhood of the protein molecule. Sorbitol and trehalose do not coherently interact with the protein. One sucrose molecule binds at the active-site cleft of the enzyme.

About this StructureAbout this Structure

1JJ1 is a Single protein structure of sequence from Gallus gallus. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.

ReferenceReference

The effect of stabilizing additives on the structure and hydration of proteins: a study involving tetragonal lysozyme., Datta S, Biswal BK, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1614-20. Epub 2001, Oct 25. PMID:11679726

Page seeded by OCA on Thu Feb 21 13:23:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1jj1.gif|left|200px]]<br /><applet load="1jj1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jj1.gif|left|200px]]<br /><applet load="1jj1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jj1, resolution 1.90&Aring;" />
 '''CRYSTAL STRUCTURE OF ORTHORHOMBIC LYSOZYME GROWN AT PH 4.6 IN PRESENCE OF 5% SORBITOL'''<br />
 ==Overview==
-In order to elucidate the effect of stabilizing additives on the structure, of proteins and the associated ordered water molecules in the hydration, shell, the crystal structures of tetragonal lysozyme grown in the presence, of sucrose, sorbitol and trehalose have been refined. Also refined are the, structures of orthorhombic and monoclinic lysozyme grown under the, conditions in which tetragonal lysozyme is normally grown. A comparison of, the two sets of structures with the structure of native tetragonal, lysozyme shows that the effect of the additives on the structure of the, protein molecule is less than that of the normal minor changes associated, with differences in molecular packing. Surprisingly, the same is true of, the effect on the hydration shell, represented by the ordered water, molecules attached to the protein. Thus, it appears that the cause of the, stabilizing effect of the additives needs to be sought outside the, immediate neighbourhood of the protein molecule. Sorbitol and trehalose do, not coherently interact with the protein. One sucrose molecule binds at, the active-site cleft of the enzyme.
+In order to elucidate the effect of stabilizing additives on the structure of proteins and the associated ordered water molecules in the hydration shell, the crystal structures of tetragonal lysozyme grown in the presence of sucrose, sorbitol and trehalose have been refined. Also refined are the structures of orthorhombic and monoclinic lysozyme grown under the conditions in which tetragonal lysozyme is normally grown. A comparison of the two sets of structures with the structure of native tetragonal lysozyme shows that the effect of the additives on the structure of the protein molecule is less than that of the normal minor changes associated with differences in molecular packing. Surprisingly, the same is true of the effect on the hydration shell, represented by the ordered water molecules attached to the protein. Thus, it appears that the cause of the stabilizing effect of the additives needs to be sought outside the immediate neighbourhood of the protein molecule. Sorbitol and trehalose do not coherently interact with the protein. One sucrose molecule binds at the active-site cleft of the enzyme.
 ==About this Structure==
-JJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JJ1 OCA].
+JJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ1 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Lysozyme]]
 [[Category: Single protein]]
-[[Category: Biswal, B.K.]]
+[[Category: Biswal, B K.]]
 [[Category: Datta, S.]]
 [[Category: Vijayan, M.]]
@@ Line 22: / Line 22: @@
 [[Category: mucopeptide n-acetylmuramyl hydrolase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:18:38 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:11 2008''