1j1h: Difference between revisions

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New page: left|200px<br /><applet load="1j1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j1h" /> '''Solution structure of a tmRNA-binding protei...
 
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[[Image:1j1h.gif|left|200px]]<br /><applet load="1j1h" size="350" color="white" frame="true" align="right" spinBox="true"  
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'''Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus'''<br />
'''Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus'''<br />


==Overview==
==Overview==
Small protein B (SmpB) is required for trans-translation, binding, specifically to tmRNA. We show here the solution structure of SmpB from an, extremely thermophilic bacterium, Thermus thermophilus HB8, determined by, heteronuclear nuclear magnetic resonance methods. The core of the protein, consists of an antiparallel beta-barrel twisted up from eight, beta-strands, each end of which is capped with the second or third helix, and the first helix is located beside the barrel. Its C-terminal sequence, (20 residues), which is rich in basic residues, shows a poorly structured, form, as often seen in isolated ribosomal proteins. The results are, discussed in relation to the oligonucleotide binding fold.
Small protein B (SmpB) is required for trans-translation, binding specifically to tmRNA. We show here the solution structure of SmpB from an extremely thermophilic bacterium, Thermus thermophilus HB8, determined by heteronuclear nuclear magnetic resonance methods. The core of the protein consists of an antiparallel beta-barrel twisted up from eight beta-strands, each end of which is capped with the second or third helix, and the first helix is located beside the barrel. Its C-terminal sequence (20 residues), which is rich in basic residues, shows a poorly structured form, as often seen in isolated ribosomal proteins. The results are discussed in relation to the oligonucleotide binding fold.


==About this Structure==
==About this Structure==
1J1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J1H OCA].  
1J1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J1H OCA].  


==Reference==
==Reference==
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[[Category: Kuramitsu, S.]]
[[Category: Kuramitsu, S.]]
[[Category: Nameki, N.]]
[[Category: Nameki, N.]]
[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Shibata, T.]]
[[Category: Shibata, T.]]
[[Category: Shirouzu, M.]]
[[Category: Shirouzu, M.]]
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[[Category: structural genomics]]
[[Category: structural genomics]]


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Revision as of 14:17, 21 February 2008

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1j1h

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Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus

OverviewOverview

Small protein B (SmpB) is required for trans-translation, binding specifically to tmRNA. We show here the solution structure of SmpB from an extremely thermophilic bacterium, Thermus thermophilus HB8, determined by heteronuclear nuclear magnetic resonance methods. The core of the protein consists of an antiparallel beta-barrel twisted up from eight beta-strands, each end of which is capped with the second or third helix, and the first helix is located beside the barrel. Its C-terminal sequence (20 residues), which is rich in basic residues, shows a poorly structured form, as often seen in isolated ribosomal proteins. The results are discussed in relation to the oligonucleotide binding fold.

About this StructureAbout this Structure

1J1H is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus., Someya T, Nameki N, Hosoi H, Suzuki S, Hatanaka H, Fujii M, Terada T, Shirouzu M, Inoue Y, Shibata T, Kuramitsu S, Yokoyama S, Kawai G, FEBS Lett. 2003 Jan 30;535(1-3):94-100. PMID:12560085

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