1io1: Difference between revisions

Revision as of 14:13, 21 February 2008

CRYSTAL STRUCTURE OF F41 FRAGMENT OF FLAGELLIN

OverviewOverview

The bacterial flagellar filament is a helical propeller constructed from 11 protofilaments of a single protein, flagellin. The filament switches between left- and right-handed supercoiled forms when bacteria switch their swimming mode between running and tumbling. Supercoiling is produced by two different packing interactions of flagellin called L and R. In switching from L to R, the intersubunit distance ( approximately 52 A) along the protofilament decreases by 0.8 A. Changes in the number of L and R protofilaments govern supercoiling of the filament. Here we report the 2.0 A resolution crystal structure of a Salmonella flagellin fragment of relative molecular mass 41,300. The crystal contains pairs of antiparallel straight protofilaments with the R-type repeat. By simulated extension of the protofilament model, we have identified possible switch regions responsible for the bi-stable mechanical switch that generates the 0.8 A difference in repeat distance.

About this StructureAbout this Structure

1IO1 is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling., Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K, Nature. 2001 Mar 15;410(6826):331-7. PMID:11268201

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OCA

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-[[Image:1io1.gif|left|200px]]<br /><applet load="1io1" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1io1, resolution 2.0&Aring;" />
 '''CRYSTAL STRUCTURE OF F41 FRAGMENT OF FLAGELLIN'''<br />
 ==Overview==
-The bacterial flagellar filament is a helical propeller constructed from, 11 protofilaments of a single protein, flagellin. The filament switches, between left- and right-handed supercoiled forms when bacteria switch, their swimming mode between running and tumbling. Supercoiling is produced, by two different packing interactions of flagellin called L and R. In, switching from L to R, the intersubunit distance ( approximately 52 A), along the protofilament decreases by 0.8 A. Changes in the number of L and, R protofilaments govern supercoiling of the filament. Here we report the, 2.0 A resolution crystal structure of a Salmonella flagellin fragment of, relative molecular mass 41,300. The crystal contains pairs of antiparallel, straight protofilaments with the R-type repeat. By simulated extension of, the protofilament model, we have identified possible switch regions, responsible for the bi-stable mechanical switch that generates the 0.8 A, difference in repeat distance.
+The bacterial flagellar filament is a helical propeller constructed from 11 protofilaments of a single protein, flagellin. The filament switches between left- and right-handed supercoiled forms when bacteria switch their swimming mode between running and tumbling. Supercoiling is produced by two different packing interactions of flagellin called L and R. In switching from L to R, the intersubunit distance ( approximately 52 A) along the protofilament decreases by 0.8 A. Changes in the number of L and R protofilaments govern supercoiling of the filament. Here we report the 2.0 A resolution crystal structure of a Salmonella flagellin fragment of relative molecular mass 41,300. The crystal contains pairs of antiparallel straight protofilaments with the R-type repeat. By simulated extension of the protofilament model, we have identified possible switch regions responsible for the bi-stable mechanical switch that generates the 0.8 A difference in repeat distance.
 ==About this Structure==
-IO1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IO1 OCA].
+IO1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IO1 OCA].
 ==Reference==
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 [[Category: Nagashima, S.]]
 [[Category: Namba, K.]]
-[[Category: Samatey, F.A.]]
+[[Category: Samatey, F A.]]
 [[Category: Vondervisz, F.]]
 [[Category: Yamamoto, M.]]
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 [[Category: flagellin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:32:05 2007''
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