1ije: Difference between revisions
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[[Image:1ije.jpg|left|200px]]<br /><applet load="1ije" size=" | [[Image:1ije.jpg|left|200px]]<br /><applet load="1ije" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ije, resolution 2.40Å" /> | caption="1ije, resolution 2.40Å" /> | ||
'''Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex'''<br /> | '''Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex'''<br /> | ||
==Overview== | ==Overview== | ||
In the elongation cycle of protein biosynthesis, the nucleotide exchange | In the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex. | ||
==About this Structure== | ==About this Structure== | ||
1IJE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with G25 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1IJE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=G25:'>G25</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Andersen, G | [[Category: Andersen, G R.]] | ||
[[Category: Kinzy, T | [[Category: Kinzy, T G.]] | ||
[[Category: Nyborg, J.]] | [[Category: Nyborg, J.]] | ||
[[Category: Pedersen, L.]] | [[Category: Pedersen, L.]] | ||
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[[Category: protein complex]] | [[Category: protein complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:31 2008'' | ||
Revision as of 14:12, 21 February 2008
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Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex
OverviewOverview
In the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex.
About this StructureAbout this Structure
1IJE is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex., Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J, Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:11373622
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