1idf: Difference between revisions
New page: left|200px<br /><applet load="1idf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1idf, resolution 2.5Å" /> '''ISOCITRATE DEHYDROGEN... |
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[[Image:1idf.jpg|left|200px]]<br /><applet load="1idf" size=" | [[Image:1idf.jpg|left|200px]]<br /><applet load="1idf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1idf, resolution 2.5Å" /> | caption="1idf, resolution 2.5Å" /> | ||
'''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''<br /> | '''ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME'''<br /> | ||
==Overview== | ==Overview== | ||
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution | Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized. | ||
==About this Structure== | ==About this Structure== | ||
1IDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http:// | 1IDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Isocitrate dehydrogenase (NADP(+))]] | [[Category: Isocitrate dehydrogenase (NADP(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bolduc, J | [[Category: Bolduc, J M.]] | ||
[[Category: Dyer, D | [[Category: Dyer, D H.]] | ||
[[Category: Junior, D | [[Category: Junior, D E.Koshland.]] | ||
[[Category: Scott, W | [[Category: Scott, W G.]] | ||
[[Category: Singer, P.]] | [[Category: Singer, P.]] | ||
[[Category: Stoddard, B | [[Category: Stoddard, B L.]] | ||
[[Category: Sweet, R | [[Category: Sweet, R M.]] | ||
[[Category: oxidoreductase (nad(a)-choh(d))]] | [[Category: oxidoreductase (nad(a)-choh(d))]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:44 2008'' | ||
