1hqc: Difference between revisions
New page: left|200px<br /><applet load="1hqc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hqc, resolution 3.2Å" /> '''STRUCTURE OF RUVB FRO... |
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[[Image:1hqc.gif|left|200px]]<br /><applet load="1hqc" size=" | [[Image:1hqc.gif|left|200px]]<br /><applet load="1hqc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1hqc, resolution 3.2Å" /> | caption="1hqc, resolution 3.2Å" /> | ||
'''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8'''<br /> | '''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8'''<br /> | ||
==Overview== | ==Overview== | ||
We report here the crystal structure of the RuvB motor protein from | We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hydrolysis. DNA is likely to interact with a large basic cleft, which encompasses the ATP-binding pocket and domain boundaries, whereas the junction-recognition protein RuvA may bind a flexible beta-hairpin protruding from the N-terminal domain. The structures of two subunits, related by a noncrystallographic pseudo-2-fold axis, imply that conformational changes of motor protein coupled with ATP hydrolysis may reflect motility essential for its translocation around double-stranded DNA. | ||
==About this Structure== | ==About this Structure== | ||
1HQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG and ADE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http:// | 1HQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADE:'>ADE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ruvb]] | [[Category: ruvb]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:03:48 2008'' | ||
Revision as of 14:03, 21 February 2008
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STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8
OverviewOverview
We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hydrolysis. DNA is likely to interact with a large basic cleft, which encompasses the ATP-binding pocket and domain boundaries, whereas the junction-recognition protein RuvA may bind a flexible beta-hairpin protruding from the N-terminal domain. The structures of two subunits, related by a noncrystallographic pseudo-2-fold axis, imply that conformational changes of motor protein coupled with ATP hydrolysis may reflect motility essential for its translocation around double-stranded DNA.
About this StructureAbout this Structure
1HQC is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as Adenosinetriphosphatase, with EC number 3.6.1.3 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:11171970
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