1god: Difference between revisions

Revision as of 13:52, 21 February 2008

MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI

OverviewOverview

Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.

About this StructureAbout this Structure

1GOD is a Single protein structure of sequence from Cerrophidion godmani. Active as Phospholipase A(2), with EC number 3.1.1.4 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani., Arni RK, Fontes MR, Barberato C, Gutierrez JM, Diaz C, Ward RJ, Arch Biochem Biophys. 1999 Jun 15;366(2):177-82. PMID:10356281

Page seeded by OCA on Thu Feb 21 12:52:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1god.gif|left|200px]]<br /><applet load="1god" size="450" color="white" frame="true" align="right" spinBox="true"
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 '''MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI'''<br />
 ==Overview==
-Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a, Ca2+-independent mechanism which does not involve catalytic activity. With, the aim of determining the structural basis for this novel activity, we, have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated, from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A, resolution by molecular replacement. The final model has been refined to a, final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with, excellent stereochemistry. godMT-II is also monomeric in the crystalline, state, and small-angle X-ray scattering results demonstrate that the, protein is monomeric in solution under fisicochemical conditions similar, to those used in the crystallographic studies.
+Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.
 ==About this Structure==
-GOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA].
+GOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Phospholipase A(2)]]
 [[Category: Single protein]]
-[[Category: Arni, R.K.]]
+[[Category: Arni, R K.]]
 [[Category: Barberato, C.]]
 [[Category: Diaz-Oreiro, C.]]
-[[Category: Fontes, M.R.M.]]
+[[Category: Fontes, M R.M.]]
-[[Category: Gutierrez, J.M.]]
+[[Category: Gutierrez, J M.]]
-[[Category: Ward, R.J.]]
+[[Category: Ward, R J.]]
 [[Category: bothrops]]
 [[Category: lys49-phospholipase a2]]
 [[Category: snake venom]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:12:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:14 2008''